![Western Blot: SLC28A1 Antibody [NBP2-30857] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/SLC28A1-Antibody-Western-Blot-NBP2-30857-img0003.jpg "Western Blot: SLC28A1 Antibody [NBP2-30857] - Staining of human small intestine shows high expression.")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: MENDPSRRRESISLTPVAKGLENMGADFLESLEEGQLPRSDLSPAEIRSSWSEAAPKPFSRWRNLQPA |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC28A1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP2-30857 | Applications | Species |
|---|---|---|
| Miller, A L, Garcia, P L Et al. Development of gemcitabine-resistant patient-derived xenograft models of pancreatic ductal adenocarcinoma. Cancer Drug Resist 2020-10-20 [PMID: 33073205] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857] - Staining in human small intestine and tonsil tissues using anti-SLC28A1 antibody. Corresponding SLC28A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SLC28A1-Antibody-Immunohistochemistry-Paraffin-NBP2-30857-img0005.jpg "Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857] - Staining in human small intestine and tonsil tissues using anti-SLC28A1 antibody. Corresponding SLC28A1 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857] - Staining of human kidney shows membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/SLC28A1-Antibody-Immunohistochemistry-Paraffin-NBP2-30857-img0002.jpg "Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857] - Staining of human kidney shows membranous positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/fullsize/SLC28A1-Antibody-Immunohistochemistry-Paraffin-NBP2-30857-img0004.jpg "Immunohistochemistry-Paraffin: SLC28A1 Antibody [NBP2-30857] - Staining of human tonsil shows low expression as expected.")
![Immunohistochemistry: SLC28A1 Antibody [NBP2-30857] - PDAC PDX models of acquired gemcitabine resistance harbored changes in expression of some gemcitabine transporters & metabolizing enzymes. Tumor tissue harvested on days 190, 120, & 179 for PA4.gemR, PA10.gemR & PA16.gemR, respectively, was immunostained for RRM1, RRM2, hCNT1, hENT1, CDA, & dCK. Tumor tissue harvested from control mice bearing gemcitabine-naïve PA4, PA10 or PA16 tumors served as controls for IHC staining experiments. Scale bar = 10 μm. Quantitation of IHC data in A is shown as expression indices below each photomicrograph (A); protein expression in gemR models that were developed by exposure to drug in vivo, compared to their respective parent tumor models. Each protein has been reported to be associated with gemcitabine resistance in models developed in vitro. ND: no difference between gemcitabine-naïve control & gemR tumors. Values in the Table were calculated by dividing the EI of each protein determined by IHC expressed by gemR/parent (control) tumors. A change of > 1.5-fold was interpreted as a real increase or decrease (B). PDAC: pancreatic ductal adenocarcinoma; PDX: patient-derived xenograft; gemR: gemcitabine resistance; IHC: immunohistochemistry Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33073205), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-30857_rabbit-polyclonal-slc28a1-antibody-31020241611479.jpg "Immunohistochemistry: SLC28A1 Antibody [NBP2-30857] - PDAC PDX models of acquired gemcitabine resistance harbored changes in expression of some gemcitabine transporters & metabolizing enzymes. Tumor tissue harvested on days 190, 120, & 179 for PA4.gemR, PA10.gemR & PA16.gemR, respectively, was immunostained for RRM1, RRM2, hCNT1, hENT1, CDA, & dCK. Tumor tissue harvested from control mice bearing gemcitabine-naïve PA4, PA10 or PA16 tumors served as controls for IHC staining experiments. Scale bar = 10 μm. Quantitation of IHC data in A is shown as expression indices below each photomicrograph (A); protein expression in gemR models that were developed by exposure to drug in vivo, compared to their respective parent tumor models. Each protein has been reported to be associated with gemcitabine resistance in models developed in vitro. ND: no difference between gemcitabine-naïve control & gemR tumors. Values in the Table were calculated by dividing the EI of each protein determined by IHC expressed by gemR/parent (control) tumors. A change of > 1.5-fold was interpreted as a real increase or decrease (B). PDAC: pancreatic ductal adenocarcinoma; PDX: patient-derived xenograft; gemR: gemcitabine resistance; IHC: immunohistochemistry Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33073205), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")