Home » SIRT6 » SIRT6 Antibodies » SIRT6 Antibody (OTI1G3)
Novus Biologicals products are now on bio-techne.com

SIRT6 Antibody (OTI1G3)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: SIRT6 Antibody (1G3) [NBP2-01310] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT6.
Western Blot: SIRT6 Antibody (1G3) [NBP2-01310] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: SIRT6 Antibody (1G3) [NBP2-01310] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT6.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
OTI1G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74198AF350
Alexa Fluor 405
NBP2-74198AF405
Alexa Fluor 488
NBP2-74198AF488
Alexa Fluor 532
NBP2-74198AF532
Alexa Fluor 594
NBP2-74198AF594
Alexa Fluor 647
NBP2-74198AF647
Alexa Fluor 700
NBP2-74198AF700
Alexa Fluor 750
NBP2-74198AF750
Biotin
NBP2-74198B
CoraFluor 1
NBP2-74198CL1
DyLight 350
NBP2-74198UV
DyLight 405
NBP2-74198V
DyLight 488
NBP2-74198G
DyLight 550
NBP2-74198R
DyLight 594
NBP2-74198DL594
DyLight 650
NBP2-74198C
DyLight 680
NBP2-74198FR
DyLight 755
NBP2-74198IR
FITC
NBP2-74198F
HRP
NBP2-74198H
Janelia Fluor 525
NBP2-74198JF525
Janelia Fluor 549
NBP2-74198JF549
Janelia Fluor 646
NBP2-74198JF646
Janelia Fluor 669
NBP2-74198JF669
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74198
Novus Biologicals offers 100% Guarantee

SIRT6 Antibody (OTI1G3) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 88-352 of human SIRT6(NP_057623) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIRT6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
Theoretical MW
38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SIRT6 Antibody (OTI1G3)

  • EC 3.5.1.-
  • NAD-dependent deacetylase sirtuin-6
  • SIR2L6sir2-related protein type 6
  • SIR2-like protein 6
  • sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
  • sirtuin 6
  • sirtuin type 6

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Sirtuin 1/SIRT1
Antibody (1F3) - BSA ...
NBP1-51641
Immunohistochemistry-Paraffin Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free
Flow Cytometry Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free
Immunocytochemistry/ Immunofluorescence Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free
(10 Publications)
Species: Hu, Mu, Pm, Rb, Rt
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, WB
Sir2.1 Antibody
NB100-1923
Western Blot Sir2.1 Antibody
(1 Review)
(2 Publications)
Species: Ce
Applications: IHC, IHC-P, IP, WB
Sirtuin 3/SIRT3
Antibody (6B2A1) - BS ...
NBP2-52563
Immunohistochemistry Whole-Mount Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free
Flow Cytometry Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free
Western Blot Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free
(1 Review)
(1 Publication)
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC, ICC/IF, IHC-WhMt, IHC, IHC-P, WB
Sirtuin 5/SIRT5
Antibody (OTI2F1) - A ...
NBP2-74199
Western Blot Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free
Western Blot Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free
Western Blot Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, KO, WB
SIRT7 Antibody
NB100-56364
Western Blot SIRT7 Antibody
Western Blot SIRT7 Antibody
(1 Publication)
Species: Hu
Applications: WB
Human, Mouse, Rat
RelA/NFkB p65 ELISA K ...
NBP2-29661
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
ELISA Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric)
(95 Publications)
Species: Hu, Mu, Rt
Applications: ELISA
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(43 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
PARP Antibody
(194C1439) - Cleaved ...
NB100-56599
Immunocytochemistry/ Immunofluorescence PARP Antibody (194C1439) - Cleaved - BSA Free
Western Blot PARP Antibody (194C1439) - Cleaved - BSA Free
Western Blot PARP Antibody (194C1439) - Cleaved - BSA Free
(10 Publications)
Species: Hu, Mu, Rt(-)
Applications: Flow-IC, Flow, ICC/IF, WB
Histone H3
Antibody - BSA Free
NB500-171
Immunohistochemistry-Paraffin Histone H3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Histone H3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Histone H3 Antibody - BSA Free
(8 Reviews)
(34 Publications)
Species: Hu, I, Mu, Rt, Xp, Ye
Applications: ChIP, IB, ICC/IF, IHC, IHC-P, Single-Cell Western, WB
SIRT4 Antibody
NB100-1406
Western Blot SIRT4 Antibody
Immunohistochemistry-Paraffin SIRT4 Antibody
Western Blot SIRT4 Antibody
(5 Publications)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
IGF-I/IGF-1
[Unconjugated]
291-G1
SDS-PAGE IGF-I/IGF-1 [Unconjugated]
Bioactivity IGF-I/IGF-1 [Unconjugated]
Mass Spectrometry IGF-I/IGF-1 [Unconjugated]
(224 Publications)
Species: Hu
Applications: BA

PBEF/Visfatin/NAMPT An
NB100-594
Western Blot PBEF/Visfatin/NAMPT Antibody
Western Blot PBEF/Visfatin/NAMPT Antibody
Immunohistochemistry-Paraffin PBEF/Visfatin/NAMPT Antibody
(10 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
HDAC9 Antibody
(OTI7G2)
NBP2-45952
Immunohistochemistry HDAC9 Antibody (OTI7G2)
Immunohistochemistry HDAC9 Antibody (OTI7G2)
Immunohistochemistry HDAC9 Antibody (OTI7G2)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(821 Publications)
Species: Hu
Applications: BA
WNK1 Antibody
[Unconjugated]
AF2849
Western Blot WNK1 Antibody [Unconjugated]
(5 Publications)
Species: Mu, Rt
Applications: WB
GRASP65 Antibody
(OTI5G8)
NBP2-02665
Immunocytochemistry/ Immunofluorescence GRASP65 Antibody (OTI5G8)
Immunocytochemistry/ Immunofluorescence GRASP65 Antibody (OTI5G8)
Flow Cytometry GRASP65 Antibody (OTI5G8)
(3 Reviews)
(2 Publications)
Species: Ca, Hu, Pm, Po, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Synaptotagmin 1
Antibody (ASV48) [Unc ...
MAB4364
Immunocytochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]
Immunohistochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]
Immunocytochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]
(2 Publications)
Species: Rt
Applications: ICC, IHC, IP, KO, WB
HIF-1 alpha
Antibody (H1alpha67)
NB100-105
Western Blot HIF-1 alpha Antibody (H1alpha67)
Flow Cytometry HIF-1 alpha Antibody (H1alpha67)
Flow Cytometry HIF-1 alpha Antibody (H1alpha67)
(45 Reviews)
(1059 Publications)
Species: Bv, Ca, Fe, Ma, Hu, Pm, Mu, Po, Pm, Rb, Rt, Sh, Xp
Applications: ChIP, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, LA, PLA, Simple Western, TCS, WB
IRS2 Antibody
(676415) [Unconjugate
MAB6347
Western Blot IRS2 Antibody (676415) [Unconjugated]
(2 Publications)
Species: Hu
Applications: WB

Publications for SIRT6 Antibody (NBP2-01310) (0)

There are no publications for SIRT6 Antibody (NBP2-01310).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SIRT6 Antibody (NBP2-01310) (0)

There are no reviews for SIRT6 Antibody (NBP2-01310). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SIRT6 Antibody (NBP2-01310) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SIRT6 Products

Research Areas for SIRT6 Antibody (NBP2-01310)

Find related products by research area.

Blogs on SIRT6

There are no specific blogs for SIRT6, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SIRT6 Antibody (OTI1G3) and receive a gift card or discount.


Bioinformatics

Gene Symbol SIRT6
Entrez