![Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-OTI2F1-Azide-and-BSA-Free-Western-Blot-NBP2-74199-img0003.jpg "Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready, KO |
| Clone | OTI2F1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human SIRT5(NP_036373) produced in HEK293 cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SIRT5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 33.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for Sirtuin 5/SIRT5 Antibody (NBP2-74199)Find related products by research area.
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| Gene Symbol | SIRT5 |
![Immunocytochemistry/Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-OTI2F1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74199-img0005.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5.")
![Immunohistochemistry: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Staining of paraffin-embedded Human Kidney tissue using anti-SIRT5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-OTI2F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74199-img0006.jpg "Immunohistochemistry: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Staining of paraffin-embedded Human Kidney tissue using anti-SIRT5 mouse monoclonal antibody.")
![Flow Cytometry: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SIRT5 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-OTI2F1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74199-img0004.jpg "Flow Cytometry: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SIRT5 antibody, and then analyzed by flow cytometry.")
![Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-OTI2F1-Azide-and-BSA-Free-Western-Blot-NBP2-74199-img0001.jpg "Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody.")
![Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Equivalent amounts of cell lysates (10ug per lane) of wild-type HeLa cells and SIRT5-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with ant](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-OTI2F1-Azide-and-BSA-Free-Western-Blot-NBP2-74199-img0002.jpg "Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) - Azide and BSA Free [NBP2-74199] - Equivalent amounts of cell lysates (10ug per lane) of wild-type HeLa cells and SIRT5-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with ant")
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![Intracellular Staining by Flow Cytometry UCP1 Antibody (536435) [Unconjugated]](https://images.novusbio.com/images2/UCP1_MAB6158_Flow_Cytometry_22032.jpg)
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![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")