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SHP-2/PTPN11 Antibody - Azide and BSA Free

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Western Blot: SHP-2/PTPN11 Antibody [NBP3-03486] - Analysis of extracts from normal (control) and PTPN11 knockout (KO) 293T cells, using SHP-2/PTPN11 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Genetic Strategies: Western Blot: SHP-2/PTPN11 Antibody [NBP3-03486] - Analysis of extracts from normal (control) and PTPN11 knockout (KO) 293T cells, using SHP-2/PTPN11 antibody at 1:1000 dilution. Secondary ...read more
Immunocytochemistry/ Immunofluorescence: SHP-2/PTPN11 Antibody [NBP3-03486] - Analysis of C6 cells using SHP-2/PTPN11 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blot: SHP-2/PTPN11 Antibody [NBP3-03486] - Analysis of extracts of various cell lines, using SHP-2/PTPN11 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. ...read more
Immunoprecipitation: SHP-2/PTPN11 Antibody [NBP3-03486] - Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug SHP-2/PTPN11 antibody (NBP3-03486). Western blot was performed from the immunoprecipitate ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-03486-20ul
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SHP-2/PTPN11 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-460 of human SHP-2/PTPN11 (NP_002825.3). MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTPN11
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • Knockout Validated
  • Western Blot 1:500-1:2000
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for SHP-2/PTPN11 Antibody - Azide and BSA Free

  • BPTP3
  • EC 3.1.3.48
  • MGC14433
  • protein tyrosine phosphatase, non-receptor type 11
  • protein tyrosine phosphatase-2
  • Protein-tyrosine phosphatase 1D
  • Protein-tyrosine phosphatase 2C
  • PTP1D
  • PTP-1D
  • PTP2C
  • PTP-2C
  • PTP2CNS1
  • PTPN11
  • SHP2
  • SHP-2
  • SHP2CFC
  • SHPTP2
  • SH-PTP2
  • SH-PTP2Noonan syndrome 1
  • SH-PTP3
  • tyrosine-protein phosphatase non-receptor type 11

Background

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SHP-2/PTPN11
Antibody - Azide and ...
NBP3-03486
SHP-2/PTPN11 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IP, KO
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Bioinformatics

Gene Symbol PTPN11