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SHMT1 Antibody

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Western Blot: SHMT1 Antibody [NBP2-20353] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Pm, Rb, RM, ShSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SHMT1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SHMT1. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Mouse (94%), Rat (94%), Sheep (95%), Primate (99%), Rhesus Macaque (99%), Bovine (95%), Rabbit (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SHMT1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (99%), Zebrafish (83%), Xenopus laevis (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for SHMT1 Antibody

  • 14 kDa protein
  • CSHMT
  • cytoplasmic serine hydroxymethyltransferase
  • EC 2.1.2.1
  • Glycine hydroxymethyltransferase
  • MGC15229
  • MGC24556
  • serine hydroxymethyltransferase 1 (soluble)
  • serine hydroxymethyltransferase, cytosolic
  • Serine methylase
  • SHMT
  • SHMT1
  • SHMTEC 2.1.2.1

Background

This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol SHMT1
Uniprot