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sFRP-5 Antibody - BSA Free

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Western Blot: SFRP5 Antibody [NB200-121] - Analysis of human SFRP5 in pancreas lysate with anti SFRP5 at a 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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sFRP-5 Antibody - BSA Free Summary

Immunogen
n/a
Specificity
Reacts with residues 25-38 [APARCEEYDYYGWQ] of human SFRP5. 92% identity bovine. 85% identity rat. 78% identity mouse.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SFRP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500 -1:1000
Application Notes
Detects a 38kDa band by Western Blot. The investigator should determine the optimal dilutions.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for sFRP-5 Antibody - BSA Free

  • frizzled-related protein 1b
  • FRP1B
  • FRP-1b
  • SARP3
  • SARP-3
  • secreted apoptosis related protein 3
  • secreted apoptosis-related protein 3
  • secreted frizzled-related protein 5
  • sFRP5
  • sFRP-5

Background

Human secreted frizzled-related protein 5 (SFRP5), which appears to act by modulating Wnt signal transduction. Wnts have been implicated in embryonic induction, cell fate determination, control of cell polarity and malignant transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for sFRP-5 Antibody (NB200-121) (0)

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Secondary Antibodies

 

Isotype Controls

Additional sFRP-5 Products

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Bioinformatics

Gene Symbol SFRP5