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Recombinant Human sFRP-3 Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human sFRP-3 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280. The ED50 for this effect is 0.05-0.25 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human sFRP-3 protein
Ala33-Asn325
Accession #
N-terminal Sequence
Ala33
Protein/Peptide Type
Recombinant Proteins
Gene
FRZB
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
39 kDa, reducing conditions
Publications
Read Publication using
7584-SF/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human sFRP-3 Protein, CF

  • FIZ
  • FREOS1
  • Frezzled
  • Fritz
  • Frizzled-related protein 1
  • frizzled-related protein
  • FRP
  • FRP-3
  • FRZB
  • Frzb1
  • FRZB-1
  • FRZB1sFRP-3
  • FRZB-PEN
  • FZRB
  • hFIZ
  • secreted frizzled-related protein 3
  • sFRP3
  • sFRP-3
  • SFRP3frezzled
  • SRFP3frizzled homolog-related

Background

Secreted Frizzled Related Protein‑3 (sFRP‑3), also known as FRZB, belongs to a family of Wnt-binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30‑35 kDa in size and contain an N‑terminal Frizzled‑like domain with 10 conserved cysteines and a Netrin-like C‑terminal domain (1‑4). Mature human sFRP‑3 shares 93% aa sequence identity with mouse and rat sFRP‑3. It neutralizes the bioactivity of Wnt‑1, ‑5a, ‑9a, Xenopus Xwnt‑8, and EGF (5‑8). sFRP‑3 regulates somitic myogenesis and osteoblast differentiation as well as morphogenesis of the cochlear epithelium, hair follicle, and cardiac atrioventricular cushion (6, 8‑10). sFRP‑3 also functions as a tumor suppressor in a variety of cancers including melanoma, medulloblastoma, soft tissue sarcoma, and androgen‑independent prostate cancer. It inhibits epithelial-mesenchymal transition, tumor growth and invasion, and the activity of MMP‑2 and MMP‑9 (7, 11‑13). In contrast, it can promote tumor growth and angiogenesis in renal cancer (14).
  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.
  4. Hoang, B. et al. (1996) J. Biol. Chem. 271:26131.
  5. Wang, S. et al. (1997) Cell 88:757.
  6. Person, A.D. et al. (2005) Dev. Biol. 278:35.
  7. Ekstrom, E.J. et al. (2011) PLoS ONE 6:e18674.
  8. Scardigli, R. et al. (2008) PLoS ONE 3:e2471.
  9. Qian, D. et al. (2007) Dev. Biol. 306:121.
  10. Chung, Y.-S. et al. (2004) J. Bone Miner. Res. 19:1395.
  11. Kongkham, P.N. et al. (2010) Oncogene 29:3017.
  12. Guo, Y. et al. (2008) Cancer Res. 68:3350.
  13. Zi, X. et al. (2005) Cancer Res. 65:9762.
  14. Hirata, H. et al. (2010) Cancer Res. 70:1896.

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Publications for sFRP-3/FRZB (7584-SF/CF)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol FRZB
Uniprot