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sFRP-1 Antibody [Janelia Fluor® 525]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-499AF350
Alexa Fluor 405
NB600-499AF405
Alexa Fluor 488
NB600-499AF488
Alexa Fluor 532
NB600-499AF532
Alexa Fluor 594
NB600-499AF594
Alexa Fluor 647
NB600-499AF647
Alexa Fluor 700
NB600-499AF700
Alexa Fluor 750
NB600-499AF750
Allophycocyanin
NB600-499APC
Biotin
NB600-499B
CoraFluor 1
NB600-499CL1
DyLight 350
NB600-499UV
DyLight 405
NB600-499V
DyLight 488
NB600-499G
DyLight 550
NB600-499R
DyLight 594
NB600-499DL594
DyLight 650
NB600-499C
DyLight 680
NB600-499FR
DyLight 755
NB600-499IR
FITC
NB600-499F
HRP
NB600-499H
Janelia Fluor 549
NB600-499JF549
Janelia Fluor 585
NB600-499JF585
Janelia Fluor 635
NB600-499JF635
Janelia Fluor 646
NB600-499JF646
Janelia Fluor 669
NB600-499JF669
PE
NB600-499PE
PerCP
NB600-499PCP
Unconjugated
NB600-499
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sFRP-1 Antibody [Janelia Fluor® 525] Summary

Immunogen
sFRP-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein. (Uniprot: Q8N474)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SFRP1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Expect cross reactivity with sFRP-1 from mouse, rat and bovine sources as only a single amino acid residue change is found within the immunogen sequence (91% positive by BLAST). Cross reactivity with sFRP-1 homologues from other sources has not been determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for sFRP-1 Antibody [Janelia Fluor® 525]

  • FRP1
  • FRP-1FrzA
  • FRPSARP-2
  • FrzA
  • SARP-2
  • SARP2sFRP-1
  • Secreted apoptosis-related protein 2
  • secreted frizzled-related protein 1
  • sFRP1
  • sFRP-1

Background

SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzled-related Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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sFRP-1 Antibody
[Janelia Fluor® ...
NB600-499JF525
sFRP-1 Antibody [Janelia Fluor® 525]
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC

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Product General Protocols

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Bioinformatics

Gene Symbol SFRP1