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Serpin E1/PAI-1 Antibody [Unconjugated]

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Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin E1/PAI-1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by ...read more
Simple Western lane view shows lysates of mouse placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI‑1 at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Serpin ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF3828
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3828-SP
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Serpin E1/PAI-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin E1/PAI-1
Leu24-Ser402
Accession # AAH54091
Specificity
Detects mouse Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Serpin E1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SERPINE1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Simple Western 12.5 ug/mL
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF3828 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin E1/PAI-1 Antibody [Unconjugated]

  • Endothelial plasminogen activator inhibitor
  • Nexin
  • PAI1
  • PAI-1
  • PAI1PAI-1
  • PAISerpin E1
  • PLANH1
  • PLANH1plasminogen activator inhibitor 1
  • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
  • Serpin E1
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serpin E1/PAI-1 Antibody (AF3828)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(6)
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Human
(1)
Mouse
(5)
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Showing Publications 1 - 9 of 9.
Publications using AF3828 Applications Species
E Duregotti, CM Reumiller, U Mayr, M Hasman, LE Schmidt, SA Burnap, K Theofilato, J Barallobre, A Beran, M Grandoch, A Viviano, M Jahangiri, M Mayr Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity Nature Communications, 2022-11-25;13(1):7269. 2022-11-25 [PMID: 36433953] (Western Blot, Mouse) Western Blot Mouse
Xue Hong, Yanni Zhou, Dedong Wang, Fuping Lyu, Tianjun Guan, Youhua Liu et al. Exogenous Wnt1 Prevents Acute Kidney Injury and Its Subsequent Progression to Chronic Kidney Disease Frontiers in Physiology 11/8/2021 [PMID: 34819873]
J Miao, J Huang, C Luo, H Ye, X Ling, Q Wu, W Shen, L Zhou Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells Physiological Reports, 2021-01-01;9(2):e14696. 2021-01-01 [PMID: 33463897] (Western Blot, Mouse) Western Blot Mouse
H Wang, J Lu, X Chen, M Schwalbe, JE Gorka, JA Mandel, J Wang, ES Goetzman, S Ranganatha, SF Dobrowolsk, EV Prochownik Acquired Deficiency of Peroxisomal Dicarboxylic Acid Catabolism is a Metabolic Vulnerability in Hepatoblastoma The Journal of Biological Chemistry, 2021-01-13;0(0):100283. 2021-01-13 [PMID: 33450224] (Western Blot, Mouse) Western Blot Mouse
Y Liu, Q Feng, J Miao, Q Wu, S Zhou, W Shen, Y Feng, FF Hou, Y Liu, L Zhou C-X-C motif chemokine receptor 4 aggravates renal fibrosis through activating JAK/STAT/GSK3&beta/&beta-catenin pathway J. Cell. Mol. Med., 2020-03-02;0(0):. 2020-03-02 [PMID: 32119183] (Western Blot, Human) Western Blot Human
J Miao, J Liu, J Niu, Y Zhang, W Shen, C Luo, Y Liu, C Li, H Li, P Yang, Y Liu, FF Hou, L Zhou Wnt/?-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction Aging Cell, 2019-07-18;0(0):e13004. 2019-07-18 [PMID: 31318148] (Western Blot, Mouse) Western Blot Mouse
Zhen Li, Lili Zhou, Yongping Wang, Jinhua Miao, Xue Hong, Fan Fan Hou et al. (Pro)renin Receptor Is an Amplifier of Wnt/ beta -Catenin Signaling in Kidney Injury and Fibrosis Journal of the American Society of Nephrology 8/1/2017 [PMID: 28270411]
Zuo Y, Chun B, Potthoff S, Kazi N, Brolin T, Orhan D, Yang H, Ma L, Kon V, Myohanen T, Rhaleb N, Carretero O, Fogo A Thymosin beta4 and its degradation product, Ac-SDKP, are novel reparative factors in renal fibrosis. Kidney Int, 2013-06-05;84(6):1166-75. 2013-06-05 [PMID: 23739235] (Western Blot, Mouse) Western Blot Mouse
Alysia Kern Lovgren, Jeffrey J. Kovacs, Ting Xie, Erin N. Potts, Yuejuan Li, W. Michael Foster et al. beta -Arrestin Deficiency Protects Against Pulmonary Fibrosis in Mice and Prevents Fibroblast Invasion of Extracellular Matrix Science Translational Medicine 3/16/2011 [PMID: 21411739]

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Bioinformatics

Gene Symbol SERPINE1
Uniprot