![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378) [NBP2-48476] - Analysis of recombinant Alpha-1-Antitrypsin, Jurkat and A549 Cell Lysate using Alpha-1-Antitrypsin Monoclonal Antibody (AAT/1378).](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-AAT-1378-Western-Blot-NBP2-48476-img0002.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378) [NBP2-48476] - Analysis of recombinant Alpha-1-Antitrypsin, Jurkat and A549 Cell Lysate using Alpha-1-Antitrypsin Monoclonal Antibody (AAT/1378).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | AAT/1378 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11618) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human Serpin A1/alpha 1-Antitrypsin protein fragment (Uniprot: P01009) |
| Localization | Cytoplasmic |
| Marker | Hepatocellular & Histiocytic Marker |
| Specificity | It recognizes a protein of 54kDa, which is identified antitrypsin (AAT). The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SERPINA1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Serpin A1/alpha 1-Antitrypsin Antibody (NBP2-48476)Find related products by research area.
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![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378) [NBP2-48476] - Analysis of human Tonsil stained with Alpha-1-Antitrypsin Monoclonal Antibody (AAT/1378).](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-AAT-1378-Immunohistochemistry-Paraffin-NBP2-48476-img0001.jpg "Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378) [NBP2-48476] - Analysis of human Tonsil stained with Alpha-1-Antitrypsin Monoclonal Antibody (AAT/1378).")
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