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Semaphorin 7A Antibody [Unconjugated]

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PBMC lymphocytes treated with 5 µg/mL PHA for 2 days (A) vs naïve PBMC lymphocytes (B) were stained with Goat Anti-Human Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2068) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and Mouse anti-Human CD3 APC-conjugated Monoclonal antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.
PBMC lymphocytes treated with 5 µg/mL PHA for 2 days (A) vs naïve PBMC lymphocytes (B) were stained with Goat Anti-Human Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2068) ...read more
HT1080 cells were stained with Goat Anti-Human Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2068, filled histogram) or isotype control antibody (Catalog # AB-108-C, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2068
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2068-SP
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Semaphorin 7A Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7A
His47-Ala648
Accession # O75326
Specificity
Detects human Semaphorin 7A in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Semaphorin 7A is observed. 
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SEMA7A
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2068 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 7A Antibody [Unconjugated]

  • CD108 antigen
  • CD108
  • CD108MGC126696
  • CDw108
  • H-SEMA-K1
  • H-Sema-L
  • JMH blood group antigen
  • JMH
  • John-Milton-Hargen human blood group Ag
  • MGC126692
  • sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A (JMH blood group)
  • sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A
  • sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A
  • Sema K1
  • Sema L
  • Sema7A
  • SEMAK1
  • SEMAL
  • SEMALJohn Milton Hagen blood group H-Sema K1
  • Semaphorin 7A
  • semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
  • semaphorin K1
  • semaphorin L
  • semaphorin-7A
  • semaphorin-K1
  • semaphorin-L

Background

Semaphorin 7A (Sema 7A), also known as Sema L, Sema K1 and CD108, is an 80 kDa glycosylphosphatidylinositol-anchored protein belonging to the Semaphorin superfamily. It binds to the beta 1 integrin subunit as well as Plexin C1. The Sema domain of human Sema 7A shares 92% and 90% amino acid sequence homology with that of the mouse and canine protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Semaphorin 7A Antibody (AF2068)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC, IHC, Immunoprecipitation, Western Blot.


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ICC
(1)
IHC
(1)
Immunoprecipitation
(1)
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(1)
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(4)
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Bioinformatics

Gene Symbol SEMA7A
Uniprot