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Plexin A3 Antibody [Unconjugated]

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Plexin A3 was detected in immersion fixed frozen sections of embryonic rat spinal cord using Goat Anti-Mouse Plexin A3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4075) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Plexin A3 was detected in immersion fixed frozen sections of embryonic rat spinal cord using Goat Anti-Mouse Plexin A3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4075) at 10 µg/mL overnight at 4 °C. ...read more
Plexin A3 was detected in immersion fixed paraffin-embedded sections of rat brain (cortex) using Goat Anti-Mouse/Rat Plexin A3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4075) at ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF4075-SP
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Plexin A3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Plexin A3
Leu34-Asp150
Accession # P70208
Specificity
Detects mouse Plexin A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A2, recombinant human (rh) Plexin B1, rhPlexin C1, and rhPlexin D1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PLXNA3
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF4075 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin A3 Antibody [Unconjugated]

  • HSSEXGENE
  • Plexin A3
  • plexin-4
  • plexin-A3
  • Plxn3
  • PLXN4
  • PLXNA3
  • Semaphorin receptor SEX
  • Sex chromosome X transmembrane protein of HGF receptor family 3
  • SEX
  • SEXPLEXIN-A3,6.3
  • XAP-6

Background

Plexin A3 (also known as SEX) is a 220 kDa member of the Plexin A subfamily of semaphorin receptors. Mature mouse Plexin A3 is predicted to be an 1853 amino acid (aa) type I transmembrane glycoprotein that contains a 1202 aa extracellular domain (ECD) and a 629 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 34-473), three plexin-semaphorin-integrin (PSI) domains (aa 492-830), and four lg-like plexin-transcription factor (IPT) repeats (aa 841-1209). Plexin A3 interacts with neuropilins 1 and 2, and binds class 3 semaphorins. Over aa 35-150, mouse Plexin A3 is 99% and 97% aa identical to rat and human Plexin A3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Plexin A3 Antibody
[Unconjugated]
AF4075
Plexin A3 Antibody [Unconjugated]
Species: Mu, Rt
Applications: WB, IHC

Publications for Plexin A3 Antibody (AF4075)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, Immunoprecipitation.


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IHC
(2)
Immunoprecipitation
(1)
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(2)
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Bioinformatics

Gene Symbol PLXNA3
Uniprot