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Semaphorin 4D/CD100 Antibody [Unconjugated]

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EL‑4 mouse lymphoblast cell line were stained with Sheep Anti-Mouse Semaphorin 4D/CD100 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5235, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
EL‑4 mouse lymphoblast cell line were stained with Sheep Anti-Mouse Semaphorin 4D/CD100 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5235, filled histogram) or control antibody (Catalog # 5‑001‑A, ...read more
Semaphorin 4D/CD100 was detected in immersion fixed frozen sections of mouse embryonic eye cup (15 d.p.c.) using Sheep Anti-Mouse Semaphorin 4D/CD100 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5235) at ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF5235
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Catalog# & Formulation Size Price
Carrier Free
AF5235-SP
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Semaphorin 4D/CD100 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100
Phe24-Thr657
Accession # NP_038688
Specificity
Detects mouse Semaphorin 4D/CD100 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 4B, and rhSemaphrin 6C is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SEMA4D
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF5235 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4D/CD100 Antibody [Unconjugated]

  • A8
  • BB18
  • CD100 antigen
  • CD100
  • CD100M-sema-G
  • COLL-4
  • FLJ33485
  • FLJ34282
  • FLJ39737
  • FLJ46484
  • immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
  • MGC169138
  • MGC169141
  • M-sema-G
  • sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
  • SEMA4D
  • SEMAJ
  • Semaphorin 4D
  • semaphorin-4D

Background

Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Sema4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Sema4D shares 87% and 93% aa identity with human and rat Sema4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Sema4D binds Plexin-B1, but also CD72 on B and dendritic cells. In developing neurons, Sema-4D assists in guidance. Sema4D is proangiogenic when expressed by tumor-associated macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Semaphorin 4D/CD100 Antibody (AF5235)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol SEMA4D
Uniprot