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SEC14L2 Antibody (OTI4H2) - Azide and BSA Free

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Western Blot: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2.
Immunohistochemistry: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SEC14L2 mouse monoclonal antibody.
Flow Cytometry: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SEC14L2 ...read more
Western Blot: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SEC14L2 monoclonal antibody.
Immunohistochemistry: SEC14L2 Antibody (OTI4H2) - Azide and BSA Free [NBP2-74051] - Staining of paraffin-embedded Human prostate tissue using anti-SEC14L2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI4H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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SEC14L2 Antibody (OTI4H2) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human SEC14L2(NP_036561) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SEC14L2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SEC14L2 Antibody (OTI4H2) - Azide and BSA Free

  • C22orf6
  • hTAP
  • KIAA1186SEC14 (S. cerevisiae)-like 2
  • KIAA1658SPFAlpha-tocopherol-associated protein
  • SEC14-like 2 (S. cerevisiae)
  • SEC14-like protein 2
  • Squalene transfer protein
  • Supernatant protein factor
  • TAP1
  • TAPMGC65053
  • tocopherol-associated protein

Background

SEC14L2 encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SEC14L2