![Immunohistochemistry-Paraffin: SCD-1 Antibody [NB100-2476] - Staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/SCD-1-Antibody-Immunohistochemistry-Paraffin-NB100-2476-img0005.jpg "Immunohistochemistry-Paraffin: SCD-1 Antibody [NB100-2476] - Staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-RIKRTGDGNYKSG, from the C Terminus of the protein sequence according to NP_005054.3. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SCD |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_005054.3. The 30kDa band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Liver shows textured cytoplam staining in hepatocytes. Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-2476 | Applications | Species |
|---|---|---|
| Zhang S, Yang Y, Shi Y. Characterization of human SCD2, an oligomeric desaturase with improved stability and enzyme activity by cross-linking in intact cells. Biochem J 2004-12-20 [PMID: 15610069] |
Secondary Antibodies |
Isotype Controls |
Research Areas for SCD-1 Antibody (NB100-2476)Find related products by research area.
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![Flow Cytometry: SCD Antibody [NB100-2476] - staining of HepG2 cells mixed in with PBL. Detected by fluorescence with PE for SCD in green and with OC515 for CD45 (CYT-450C) in red.](http://images.novusbio.com/fullsize/SCD-Antibody-Flow-Cytometry-NB100-2476-img0004.jpg "Flow Cytometry: SCD Antibody [NB100-2476] - staining of HepG2 cells mixed in with PBL. Detected by fluorescence with PE for SCD in green and with OC515 for CD45 (CYT-450C) in red.")
![Immunohistochemistry-Paraffin: SCD Antibody [NB100-2476] - (5ug/ml) staining Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/SCD-Antibody-Immunohistochemistry-Paraffin-NB100-2476-img0002.jpg "Immunohistochemistry-Paraffin: SCD Antibody [NB100-2476] - (5ug/ml) staining Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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