Western Blot: SAP130 Antibody [NB100-1077] - Staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using ...read more
WB: Approx. 150 kDa band observed in HeLa nuclear lysates (calculated MW of 136 kDa band according to NP_036558.3). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 23594796). Use in ELISA reported in scientific literature (PMID: 22564223).
Theoretical MW
136 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.