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Recombinant Human RPA2 His Protein

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SDS-Page: Recombinant Human RPA2 Protein [NBP1-50878] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain, 31.7 kDa (293aa) confirmed by MALDI-TOF

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

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Recombinant Human RPA2 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-270 of Human RPA2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMWNSGFE SYGSSSYGGA GGYTQSPGGF GSPAPSQAEK KSRARAQHIV PCTISQLLSA TLVDEVFRIG NVEISQVTIV GIIRHAEKAP TNIVYKIDDM TAAPMDVRQW VDTDDTSSEN TVVPPETYVK VAGHLRSFQN KKSLVAFKIM PLEDMNEFTT HILEVINAHM VLSKANSQPS AGRAPISNPG MSEAGNFGGN SFMPANGLTV AQNQVLNLIK ACPRPEGLNF QDLKNQLKHM SVSSIKQAVD FLSNEGHIYS TVDDDHFKST DAE

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
RPA2
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl, 2mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human RPA2 His Protein

  • REPA2
  • Replication factor A protein 2
  • replication protein A 32 kDa subunit
  • Replication protein A 34 kDa subunit
  • replication protein A2 (32kD)
  • replication protein A2, 32kDa
  • RF-A protein 2
  • RP-A p32
  • RP-A p34
  • RPA2
  • RPA32
  • RPA34

Background

Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. The C-terminus of RPA2 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, this protein binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA2 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3. Recombinant human RPA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol RPA2