![Western Blot: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Azide-and-BSA-Free-Western-Blot-NBP2-73935-img0003.jpg "Western Blot: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.")
| Reactivity | Hu, Mu, Rt, Pm, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | OTI9A1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RPA2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 29.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for RPA2 Antibody (NBP2-73935)Find related products by research area.
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Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD) Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm. Transportin 1 is co... Read full blog post. |
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| Gene Symbol | RPA2 |
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73935-img0006.jpg "Immunocytochemistry/Immunofluorescence: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2.")
![Immunohistochemistry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Staining of paraffin-embedded Human thyroid tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73935-img0010.jpg "Immunohistochemistry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Staining of paraffin-embedded Human thyroid tissue using anti-RPA2 mouse monoclonal antibody.")
![Flow Cytometry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Analysis of Jurkat cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73935-img0005.jpg "Flow Cytometry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Analysis of Jurkat cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Azide-and-BSA-Free-Western-Blot-NBP2-73935-img0001.jpg "Western Blot: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Western Blot: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Ajuba associates with RPA in HTC75 and IMR90 cells. IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: tota](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Azide-and-BSA-Free-Western-Blot-NBP2-73935-img0002.jpg "Western Blot: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Ajuba associates with RPA in HTC75 and IMR90 cells. IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: tota")
![Immunohistochemistry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73935-img0007.jpg "Immunohistochemistry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody.")
![Immunohistochemistry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Staining of paraffin-embedded Human colon tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73935-img0008.jpg "Immunohistochemistry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Staining of paraffin-embedded Human colon tissue using anti-RPA2 mouse monoclonal antibody.")
![Immunohistochemistry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Staining of paraffin-embedded Human prostate tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73935-img0009.jpg "Immunohistochemistry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Staining of paraffin-embedded Human prostate tissue using anti-RPA2 mouse monoclonal antibody.")
![Flow Cytometry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Analysis of Hela cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73935-img0004.jpg "Flow Cytometry: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - Analysis of Hela cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Immunoprecipitation: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Azide-and-BSA-Free-Immunoprecipitation-NBP2-73935-img0011.jpg "Immunoprecipitation: RPA2 Antibody (OTI9A1) - Azide and BSA Free [NBP2-73935] - (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.")
![Western Blot Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/Histone_H2AX_AF2288_Western_Blot_5182.jpg)
![Simple Western Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/16395.jpg)
![Immunocytochemistry Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/Histone_H2AX_AF2288_Immunocytochemistry__Immunofluorescence_16920.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")