![Western Blot: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of RPA3 expression in transfected 293T cell line by RPA3 monoclonal antibody (M01), clone 1F4.Lane 1: RPA3 transfected lysate(13.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RPA14-Antibody-1F4-Western-Blot-H00006119-M01-img0009.jpg "Western Blot: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of RPA3 expression in transfected 293T cell line by RPA3 monoclonal antibody (M01), clone 1F4.Lane 1: RPA3 transfected lysate(13.6 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, KD |
| Clone | 1F4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | RPA3 (AAH05264, 12 a.a. ~ 121 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. INAGMLAQFIDKPVCFVGRLEKIHPTGKMFILSDGEGKNGTIELMEPLDEEISGIVEVVGRVTAKATILCTSYVQFKEDSHPFDLGLYNEAVKIIHDFPQFYPLGIVQHD |
| Localization | Nuclear |
| Specificity | RPA3 - replication protein A3, 14kDa |
| Isotype | IgG1 Lambda |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RPA3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Immunocytochemistry/ Immunofluorescence RPA2 [p Ser4, p Ser8] Antibody](https://images.novusbio.com/images/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-23017-img0046.jpg)
![Western Blot RPA2 [p Ser4, p Ser8] Antibody](https://images.novusbio.com/images/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0047.jpg)
![Immunoprecipitation RPA2 [p Ser4, p Ser8] Antibody](https://images.novusbio.com/images/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunoprecipitation-NBP1-23017-img0048.jpg)
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
| Publication using H00006119-M01 | Applications | Species |
|---|---|---|
| Efi F, Nick G, Nikolaos A et al. Replication Protein A (RPA1, RPA2 and RPA3) expression in gastric cancer: correlation with clinicopathologic parameters and patients' survival. J BUON. 2020-05-15 [PMID: 32862594] |
Secondary Antibodies |
Isotype Controls |
Research Areas for RPA14 Antibody (H00006119-M01)Find related products by research area.
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![Immunohistochemistry-Paraffin: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of monoclonal antibody to RPA3 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RPA14-Antibody-1F4-Immunohistochemistry-Paraffin-H00006119-M01-img0006.jpg "Immunohistochemistry-Paraffin: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of monoclonal antibody to RPA3 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.")
![Western Blot: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of RPA3 over-expressed 293 cell line, cotransfected with RPA3 Validated Chimera RNAi ( Cat # H00006119-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RPA3 monoclonal antibody (M01), clone 1F4 (Cat # H00006119-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/RPA14-Antibody-1F4-Western-Blot-H00006119-M01-img0007.jpg "Western Blot: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of RPA3 over-expressed 293 cell line, cotransfected with RPA3 Validated Chimera RNAi ( Cat # H00006119-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RPA3 monoclonal antibody (M01), clone 1F4 (Cat # H00006119-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: RPA14 Antibody (1F4) [H00006119-M01] - RPA3 monoclonal antibody (M01), clone 1F4 Analysis of RPA3 expression in HL-60.](http://images.novusbio.com/fullsize/RPA14-Antibody-1F4-Western-Blot-H00006119-M01-img0008.jpg "Western Blot: RPA14 Antibody (1F4) [H00006119-M01] - RPA3 monoclonal antibody (M01), clone 1F4 Analysis of RPA3 expression in HL-60.")
