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RelA/NFkB p65 Antibody (112A1021) [Biotin]

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Western Blot: RelA/NFkB p65 Antibody (112A1021) [Biotin] [NB100-56055] - Analysis ofRelA/NFkB p65 Antibody (112A1021) [Biotin]using NB100-56055 at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, IP
Clone
112A1021
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

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RelA/NFkB p65 Antibody (112A1021) [Biotin] Summary

Immunogen
This NfkB p65 antibody was raised against a synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RELA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1ul/1 million cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Readout System
Control
NFkB p105/p50 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-56055 in the following applications:

Publications
Read Publications using
NB100-56055 in the following applications:

Reactivity Notes

Predicted to react with Bovine, Gorilla, New World Monkey and Porcine.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for RelA/NFkB p65 Antibody (112A1021) [Biotin]

  • MGC131774
  • nf kb p65
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65
  • p65RelA
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RelA/NFkB p65 Antibody (NB100-56055)(4)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC-P.


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IHC-P
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Review for RelA/NFkB p65 Antibody (NB100-56055) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56055:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Mouse 09/14/2021
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Summary

ApplicationWestern Blot
Sample TestedNIH 3T3 cell lysate
SpeciesMouse
Lot112A1021

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RelA/NFkB p65 Antibody (NB100-56055) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional RelA/NFkB p65 Products

Research Areas for RelA/NFkB p65 Antibody (NB100-56055)

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Blogs on RelA/NFkB p65.

Killing two birds with one stone: Treating inflammation and cancer by inhibiting prolyl-4-hydroxylase-1
By Jamshed Arslan Pharm.D. The cell’s oxygen-sensing machinery comprises prolyl-4-hydroxylases (P4Hs 1-3, PHDs 1-3, or EGLN 1-3) and their canonical target hypoxia-inducible factors (HIFs). When oxygen levels ...  Read full blog post.

The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit...  Read full blog post.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
09/14/2021
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol RELA