Recombinant Rat IFN-beta Protein

Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. As there is no international Rat IFN-beta standard, units are determined with respect to the international reference standard for mouse interferon alpha/beta (mouse IFN-alpha / beta ) provided by the National Institutes of Health [see Pestka, S. (1986) “Interferon Standards and General Abbreviations, ” in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14- 23]. Units of activity measured on rat epithelial-like (RATEC) cells with VSV.

Chinese Hamster Ovary cell line, CHO-derived rat IFN-beta protein

Recombinant Proteins

Ifnb1

<1.0 EU per 1 μg of the protein by the LAL method.

19.68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.