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Recombinant Rat CX3CL1/Fractalkine (Chemokine Domain)

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price
Carrier Free
537-FT-025/CF
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Recombinant Rat CX3CL1/Fractalkine (Chemokine Domain) Summary

Preparation
Method
Lyophilized from a 0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED50 for this effect is 3-18 ng/mL.
Source
E. coli-derived rat CX3CL1/Fractalkine protein
Gln25-Gly100
Accession #
N-terminal Sequence
Gln25
Protein/Peptide Type
Recombinant Proteins
Gene
CX3CL1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
8.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
537-FT in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CX3CL1/Fractalkine (Chemokine Domain)

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X3-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Rat CX3CL1 cDNA encodes a 393 amino acid (aa) residue precursor protein with two alternative (21 aa or 24 aa residue) putative signal peptides, a 76 aa residue globular chemokine domain, a 238 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 36 aa residue cytoplasmic domain. The extracellular domain of CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, rat CX3CL1 shares a high degree of amino sequence homology (83% sequence identity) with human and mouse CX3CL1. CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up‑regulated on activated endothelial cells. Membrane‑bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T‑lymphocytes but not monocytes. CX3CR1, previously named V28 or chemokine beta receptor‑like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind fractalkine with high-affinity.

  1. Kledal, T.N. et al. (1998) FEBS Lett. 441:209.
  2. Combadiere, C. et al. (1998) J. Biol. Chem. 273:23799.
  3. Harrison, J.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10896.
  4. Rossi, D.L. et al. (1998) Genomics 47:163.

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Recombinant Rat
CX3CL1/Fractalkine (C ...
537-FT
Recombinant Rat CX3CL1/Fractalkine (Chemokine Domain)
Species: Rt
Applications: Bioactivity

Publications for CX3CL1/Fractalkine (537-FT)(5)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(3)
In Vivo
(2)
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Rat
(5)
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Showing Publications 1 - 5 of 5.
Publications using 537-FT Applications Species
M Jiang, H Xie, C Zhang, T Wang, H Tian, L Lu, JY Xu, GT Xu, L Liu, J Zhang Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy Journal of Cellular and Molecular Medicine, 2022-01-11;0(0):. 2022-01-11 [PMID: 35023309] (Bioassay, Rat) Bioassay Rat
Clark A, Gruber-Schoffnegger D, Drdla-Schutting R, Gerhold K, Malcangio M, Sandkuhler J Selective activation of microglia facilitates synaptic strength. J Neurosci, 2015-03-18;35(11):4552-70. 2015-03-18 [PMID: 25788673] (Bioassay, Rat) Bioassay Rat
Husberg C, Nygard S, Finsen AV, Damas JK, Frigessi A, Oie E, Waehre A, Gullestad L, Aukrust P, Yndestad A, Christensen G Cytokine expression profiling of the myocardium reveals a role for CX3CL1 (fractalkine) in heart failure. J. Mol. Cell. Cardiol., 2008-05-28;45(2):261-9. 2008-05-28 [PMID: 18585734] (Bioassay, Rat) Bioassay Rat
Chen X, Geller EB, Rogers TJ, Adler MW The chemokine CX3CL1/fractalkine interferes with the antinociceptive effect induced by opioid agonists in the periaqueductal grey of rats. Brain Res., 2007-03-28;1153(0):52-7. 2007-03-28 [PMID: 17459345] (In Vivo, Rat) In Vivo Rat
Zhuang ZY, Kawasaki Y, Tan PH, Wen YR, Huang J, Ji RR Role of the CX3CR1/p38 MAPK pathway in spinal microglia for the development of neuropathic pain following nerve injury-induced cleavage of fractalkine. Brain Behav. Immun., 2006-12-15;21(5):642-51. 2006-12-15 [PMID: 17174525] (In Vivo, Rat) In Vivo Rat

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Bioinformatics

Gene Symbol CX3CL1
Uniprot