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Recombinant Porcine IL-4 Protein

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Summary
Reactivity PoSpecies Glossary
Applications Bioactivity

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Recombinant Porcine IL-4 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.15‑0.75 ng/mL.
Source
E. coli-derived porcine IL-4 protein
His25-Cys133, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL4
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
654-P4 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Porcine IL-4 Protein

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • IL4E12
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Porcine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature porcine IL-4 shares 78%, 59%, 41%, and 41% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human IL-4 is active on porcine vascular endothelial cells (5). IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, 
-15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8, 11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Bailey, M. et al. (1993) Biochim. Biophys. Acta 1171:328.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Stocker, C.J. et al. (2000) J. Immunol. 164:3309.
  6. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  7. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  8. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  9. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  10. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  11. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  12. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

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Publications for IL-4 (654-P4)(18)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), ELISA Standard.


Filter By Application
Bioassay
(13)
Cell Culture
(1)
Differentiation
(2)
ELISA (Standard)
(2)
ELISA Standard
(1)
All Applications
Filter By Species
Porcine
(17)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using 654-P4 Applications Species
Z Oreskovic, L Levá, K Chlebová, K Hlavová, R Tesa?ík, J Gebauer, M Faldyna Effects of IFNgamma and IL4 rich microenvironment on porcine monocyte-derived dendritic cell activation in vitro Research in Veterinary Science, 2022-02-06;145(0):54-62. 2022-02-06 [PMID: 35168110] (Bioassay, Porcine) Bioassay Porcine
L Zheng, F Zhao, J Ru, L Liu, Z Wang, N Wang, X Shu, Z Wei, H Guo Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells Viruses, 2021-10-26;13(11):. 2021-10-26 [PMID: 34834964] (Bioassay, Porcine) Bioassay Porcine
B Hamid, J Schlosser-, L Bechtold, F Ebner, S Rausch, S Hartmann Early Immune Initiation by Porcine Cells following Toxoplasma gondii Infection versus TLR Ligation Microorganisms, 2021-08-28;9(9):. 2021-08-28 [PMID: 34576723] (Bioassay, Porcine) Bioassay Porcine
X Qu, C Neuhoff, MU Cinar, M Pröll, E Tholen, D Tesfaye, M Hölker, K Schellande, MJ Uddin Epigenetic Modulation of TLR4 Expression by Sulforaphane Increases Anti-Inflammatory Capacity in Porcine Monocyte-Derived Dendritic Cells Biology, 2021-05-31;10(6):. 2021-05-31 [PMID: 34072812] (Cell Culture, Porcine) Cell Culture Porcine
D Zheng, Z Wang, L Sui, Y Xu, L Wang, X Qiao, W Cui, Y Jiang, H Zhou, L Tang, Y Li Lactobacillus johnsonii activates porcine monocyte derived dendritic cells maturation to modulate Th cellular immune response Cytokine, 2021-05-22;144(0):155581. 2021-05-22 [PMID: 34029942] (Bioassay, Porcine) Bioassay Porcine
SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines MethodsX, 2019-05-17;6(0):1218-1227. 2019-05-17 [PMID: 31193967] (ELISA Standard, Porcine) ELISA Standard Porcine
C Linard, M Brachet, B L'homme, C Strup-Perr, E Busson, M Bonneau, JJ Lataillade, E Bey, M Benderitte Long-term effectiveness of local BM-MSCs for skeletal muscle regeneration: a proof of concept obtained on a pig model of severe radiation burn Stem Cell Res Ther, 2018-11-08;9(1):299. 2018-11-08 [PMID: 30409227] (Bioassay, Porcine) Bioassay Porcine
G Franzoni, SP Graham, G Sanna, P Angioi, MS Fiori, A Anfossi, M Amadori, S Dei Giudic, A Oggiano Interaction of porcine monocyte-derived dendritic cells with African swine fever viruses of diverse virulence Vet. Microbiol., 2018-02-18;216(0):190-197. 2018-02-18 [PMID: 29519515] (Differentiation, Differentiation, Porcine) Differentiation, Differentiation Porcine
H Singleton, SP Graham, JP Frossard, KB Bodman-Smi, F Steinbach Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10 Virology, 2018-03-02;0(0):. 2018-03-02 [PMID: 29502802] (Bioassay, Porcine) Bioassay Porcine
V Ahlberg, B Hjertner, P Wallgren, S Hellman, K Lövgren Be, C Fossum Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs Vet. Res., 2017-05-22;48(1):30. 2017-05-22 [PMID: 28532492] (Bioassay, Porcine) Bioassay Porcine
Show All 18 Publications.

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Bioinformatics

Gene Symbol IL4
Uniprot