Home » Tryptase-5/Prss32 » Tryptase-5/Prss32 Proteins and Enzymes » Recombinant Mouse Tryptase-5/Prss32 Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Mouse Tryptase-5/Prss32 Protein, CF

 Submit a Review

Images

 
There are currently no images for Tryptase-5/Prss32 (2634-SE).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Mouse Tryptase-5/Prss32 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Arg-ThioBenzyl ester (Z-R-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >400 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived mouse Tryptase-5/Prss32 protein
Ser20-Arg304, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ser20
Structure / Form
Pro form
Protein/Peptide Type
Recombinant Enzymes
Gene
Prss32
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40 kDa doublet, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile 50 mM Tris, 10 mM CaCl2, 150 mM NaCl and 0.05% Brij-35 pH 7.5.
Assay Procedure
  • Activation Buffer: (TCNB) 50 mM Tris, 0.15 M NaCl, 10 mM CaCl2, 0.05% Brij-35, pH 7.5
  • Assay Buffer: 50 mM Tris, 0.25 M NaCl, pH 8.0
  • Recombinant Mouse Tryptase‑5/Prss32 (rmTPS-5) (Catalog # 2634-SE)
  • Bacterial Thermolysin (Thermolysin) (Catalog # 3097-ZN)
  • 1,10 Phenanthroline (Sigma, Catalog # 320056), 0.6 M stock in DMSO
  • Substrate: Z-Arg-SBzl (SM Biochemicals, Catalog # SMSB01), 10 mM stock in DMSO
  • 5,5’Dithio-bis(2-nitrobenzoic acid) (DTNB) (Sigma Catalog # D-8130)
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rmTPS-5 to 100 µg/mL with Activation Buffer.
  2. Dilute Thermolysin to 0.4 µg/mL with Activation Buffer.
  3. Mix equal volumes of 100 µg/mL rmTPS-5 and 0.4 µg/mL Thermolysin for final concentrations of 50 µg/mL and 0.2 µg/mL, respectively.
  4. Incubate at 37 °C for 1 hour.
  5. Stop the reaction with 10 mM 1,10 Phenanthroline.
  6. Dilute activated rmTPS-5 to 4 ng/µL in Assay Buffer.
  7. Dilute Substrate to 200 µM in Assay Buffer with 200 µM of DTNB.
  8. Load 50 µL of the 4 ng/µL rmTPS-5 in a clear plate and start the reaction by adding 50 µL of the Substrate/DTNB mixture to wells. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL Substrate mix without any rmTPS-5.
  9. Read at 405 nm (Absorbance), in kinetic mode for 5 minutes.
  10. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank 
     **Using the extinction coefficient 13260 M-1cm-1 
     ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD Per Well:
  • rmTPS-5: 0.20 µg
  • DTNB: 100 µM
  • Substrate: 100 µM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Tryptase-5/Prss32 Protein, CF

  • EOS
  • Prss32
  • Tryptase5
  • Tryptase-5

Background

Tryptase-5 is encoded by Prss32, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse Tryptase-5 consists of 331 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 53), a catalytic domain (54 to 304), and a C-terminal hydrophobic peptide (residues 305 to 331). The mRNA is expressed in smooth muscle, eye, stomach, uterus and lymph node. Apparently, Prss32 does not seem to have a counterpart in the human genome. The mouse Tryptase-5 (residues 20 to 304) was expressed in the NS0 cells with a foreign signal peptide. The protein was secreted and purified. After being treated by thermolysin, the enzyme is active against a peptide substrate as described in the Activity Assay Protocol.

  1. Wong, G. W. et al. (2004) J. Biol. Chem. 279:2438.

Customers Who Viewed This Item Also Viewed...

IL-5 [HRP]
M5000
N/A IL-5 [HRP]
N/A IL-5 [HRP]
(92 Publications)
Species: Mu
Applications: ELISA
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
RNASE3 Antibody
NBP2-33778
Western Blot RNASE3 Antibody
Immunohistochemistry-Paraffin RNASE3 Antibody
Immunohistochemistry-Paraffin RNASE3 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
Ovalbumin Antibody
NB600-922
Western Blot Ovalbumin Antibody
Western Blot Ovalbumin Antibody
Western Blot Ovalbumin Antibody
(10 Publications)
Species: Ch
Applications: ELISA, IHC, Single-Cell Western, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
ABAT Antibody
NBP2-21598
Immunocytochemistry/ Immunofluorescence ABAT Antibody
Western Blot ABAT Antibody
Immunohistochemistry-Paraffin ABAT Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Ikaros/IKZF1
Antibody [Unconjugate
AF4984
Western Blot Ikaros/IKZF1 Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) Ikaros/IKZF1 Antibody [Unconjugated]
Simple Western Ikaros/IKZF1 Antibody [Unconjugated]
(2 Publications)
Species: Hu
Applications: ChIP, CyTOF-ready, ICC, ICFlow, Simple Western, WB
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(455 Publications)
Species: Hu
Applications: BA
IL-13 [Biotin]
DY413
N/A IL-13 [Biotin]
(149 Publications)
Species: Mu
Applications: ELISA
NDUFB6 Antibody
NBP1-92172
Immunohistochemistry-Paraffin NDUFB6 Antibody
Western Blot NDUFB6 Antibody
Immunocytochemistry/ Immunofluorescence NDUFB6 Antibody
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
GM-CSF
[Unconjugated]
7954-GM/CF
Bioactivity GM-CSF [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IL-3 [Unconjugated]
203-IL
SDS-PAGE IL-3 [Unconjugated]
Bioactivity IL-3 [Unconjugated]
SEC-MALS IL-3 [Unconjugated]
(217 Publications)
Species: Hu
Applications: BA
GM-CSF
[Unconjugated]
7954-GM/CF
Bioactivity GM-CSF [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
Sialin/SLC17A5
Antibody
NBP2-20383
Immunohistochemistry-Paraffin Sialin/SLC17A5 Antibody
Western Blot Sialin/SLC17A5 Antibody
Immunocytochemistry/ Immunofluorescence Sialin/SLC17A5 Antibody
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
CCL11/Eotaxin [HRP]
MME00
N/A CCL11/Eotaxin [HRP]
N/A CCL11/Eotaxin [HRP]
(57 Publications)
Species: Mu
Applications: ELISA
ICAM-1/CD54
Antibody [Unconjugate
AF796
Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
(6 Reviews)
(88 Publications)
Species: Mu
Applications: AdBlk, IHC, WB
RanGAP1 Antibody
(OTI1B4)
NBP2-02623
Flow Cytometry RanGAP1 Antibody (OTI1B4)
Flow Cytometry RanGAP1 Antibody (OTI1B4)
Flow Cytometry RanGAP1 Antibody (OTI1B4)
(1 Publication)
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB

SLC6A4/5-HTTLPR/Seroto ...
H00006532-D01P
Flow Cytometry SLC6A4/5-HTTLPR/Serotonin transporter Antibody
Western Blot SLC6A4/5-HTTLPR/Serotonin transporter Antibody
Western Blot SLC6A4/5-HTTLPR/Serotonin transporter Antibody
(1 Review)
(1 Publication)
Species: Hu, Mu
Applications: WB
Recombinant Mouse
Tryptase-5/Prss32 Pro ...
2634-SE
Recombinant Mouse Tryptase-5/Prss32 Protein, CF
Species: Mu
Applications: Enzyme Activity

Publications for Tryptase-5/Prss32 (2634-SE) (0)

There are no publications for Tryptase-5/Prss32 (2634-SE).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Tryptase-5/Prss32 (2634-SE) (0)

There are no reviews for Tryptase-5/Prss32 (2634-SE). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Tryptase-5/Prss32 (2634-SE) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Tryptase-5/Prss32 Products

Blogs on Tryptase-5/Prss32

There are no specific blogs for Tryptase-5/Prss32, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Tryptase-5/Prss32 Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol Prss32
Uniprot