Recombinant Mouse IGFBP-3 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Insulin-like growth factor binding protein-3 (IGFBP-3) is one of six members of the insulin-like growth factor (IGF) binding protein superfamily which function to modulate the biological activity of IGF (1). IGFBP-3 is the major binding protein of IGF where it exists in circulation as a ternary complex with the acid-labile subunit (ALS) (2). Like other IGFBP members, IGFBP-3 includes a cysteine-rich c-terminal domain, a highly variable central linker domain, and another N-terminal cysteine-rich domain (2, 3). Mouse IGFBP-3 cDNA encodes a 291 amino acid (aa) precursor protein with a 27 aa signal peptide that is processed to generate the 264 aa mature protein. Mature mouse IGFBP-3 shares 82% and 95% aa sequence identity with human and rat IGFBP-3, respectively. Post-translational glycosylation and phosphorylation of IGFBP-3 modifies the affinities of the binding protein. Proteolysis of IGFBP-3 by tissue plasminogen activator (tPA), a disintegrin and metaloproteases (ADAMs), and prostate specific antigen (PSA) contributes to IGFBP-3 degradation or a reduction in its affinity for IGF (4-6). The majority of soluble IGFBP-3 found in circulation is secreted from hepatic non-parenchymal cells. IGFBP-3 expression can be modulated by p53 as well as by various cytokines and growth factors (7, 8). In addition to its role in stabilizing and transporting circulating IGF, IGFBP-3 has been shown to potentiate EGF-EGFR-mediated cell growth through the activation of sphingosine kinase1 (SPHK1) and sphingosin-1-phosphate (S1P) (9, 10). IGFBP-3 has also been shown to modulate adipogenesis (11). Binding of IGFBP-3 to non-IGF-related ligands has been shown to regulate TGF-beta signaling, DNA damage, apoptosis, autophagy, and gene transcription (12). Interactions with non-IGF-related ligands is thought to contribute, in part, to the dichotomous stimulatory and inhibitory effects of IGFBP-3 on cell growth (2).

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, Cell Culture, ELISA (Standard).

Showing Publications 1 - 8 of 8.

Publications using 775-B3	Applications	Species
CJ Green, M Span, MH Rayhanna, M Perera, ML Day Insulin-like Growth Factor Binding Protein 3 Increases Mouse Preimplantation Embryo Cleavage Rate by Activation of IGF1R and EGFR Independent of IGF1 Signalling Cells, 2022-11-24;11(23):. 2022-11-24 [PMID: 36497022] (Cell Culture, Mouse)	Cell Culture	Mouse
A Hiroto, WK Kim, A Pineda, Y He, DH Lee, V Le, AW Olson, J Aldahl, CH Nenninger, AJ Buckley, GQ Xiao, J Geradts, Z Sun Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis Nature Communications, 2022-11-02;13(1):6552. 2022-11-02 [PMID: 36323713] (Bioassay, Mouse)	Bioassay	Mouse
S Chen, J Jing, Y Yuan, J Feng, X Han, Q Wen, TV Ho, C Lee, Y Chai Runx2+ Niche Cells Maintain Incisor Mesenchymal Tissue Homeostasis through IGF Signaling Cell Rep, 2020-08-11;32(6):108007. 2020-08-11 [PMID: 32783935] (Bioassay, Cell Culture, Mouse)	Bioassay, Cell Culture	Mouse
EZH1 and EZH2 promote skeletal growth by repressing inhibitors of chondrocyte proliferation and hypertrophy Nat Commun, 2016-11-29;7(0):13685. 2016-11-29 [PMID: 27897169] (Bioassay, Mouse)	Bioassay	Mouse
Mehta HH, Gao Q, Galet C IGFBP-3 is a metastasis suppression gene in prostate cancer. Cancer Res., 2011-06-22;71(15):5154-63. 2011-06-22 [PMID: 21697285] (ELISA (Standard))	ELISA (Standard)
Yamada PM, Mehta HH, Hwang D Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation. Endocrinology, 2010-10-06;151(12):5741-50. 2010-10-06 [PMID: 20926583] (ELISA (Standard))	ELISA (Standard)
Raina K, Blouin MJ, Singh RP, Majeed N, Deep G, Varghese L, Glode LM, Greenberg NM, Hwang D, Cohen P, Pollak MN, Agarwal R Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model. Cancer Res., 2007-11-15;67(22):11083-91. 2007-11-15 [PMID: 18006855] (ELISA (Standard))	ELISA (Standard)
Yakar S, Bouxsein ML, Canalis E, Sun H, Glatt V, Gundberg C, Cohen P, Hwang D, Boisclair Y, LeRoith D, Rosen CJ The ternary IGF complex influences postnatal bone acquisition and the skeletal response to intermittent parathyroid hormone. J. Endocrinol., 2006-05-01;189(2):289-99. 2006-05-01 [PMID: 16648296] (ELISA (Standard))	ELISA (Standard)

Array 775-B3

• IGFBP-3 Antibodies
• IGFBP-3 Antibody Pair
• IGFBP-3 ELISA Kits
• IGFBP-3 Lysate
• IGFBP-3 Protein
• IGFBP-3 Proteins and Enzymes
• IGFBP-3 ELISAs