Novus Biologicals products are now on bio-techne.com

Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF

Images

 
There are currently no images for CD40/TNFRSF5 (1215-CD).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD40/TNFRSF5 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse CD40 Ligand/TNFSF5 (Catalog # 8230-CL) that produces 50% optimal binding is found to be 100-600 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD40/TNFRSF5 protein
Mouse CD40
(Val24-Arg193)
Accession # P27512
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Val24
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Cd40
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
46 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
58-61 kDa, reducing conditions
Publications
Read Publications using
1215-CD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF

  • B cell surface antigen CD40
  • B-cell surface antigen CD40
  • Bp50B cell-associated molecule
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • CD40 type II isoform
  • CD40
  • CD40L receptor
  • CDw40
  • MGC9013
  • nerve growth factor receptor-related B-lymphocyte activation molecule
  • p50
  • TNFRSF5
  • TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
  • tumor necrosis factor receptor superfamily member 5
  • tumor necrosis factor receptor superfamily, member 5

Background

CD40, also known as TNFRSF5, is a 32 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily. Mature mouse CD40 consists of a 174 amino acid (aa) extracellular domain, a transmembrane domain, and a 74 aa cytoplasmic domain (1, 2). The extracellular domain of mouse CD40 shares 58% and 81% aa sequence identity with human and rat CD40, respectively. Multiple mouse CD40 splice variants have been identified including soluble isoforms. CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (3, 4). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of intracellular signaling in both CD40 and CD40 Ligand expressing cells (5). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (6, 7). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (8, 9). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (10, 11). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (12, 13).

 

  1. Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620.
  2. Torres, R.M. et al. (1992) J. Immunol. 149:2641.
  3. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
  4. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  5. Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  6. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
  7. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
  8. Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
  9. Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
  10. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
  11. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
  12. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
  13. Hassan, G.S. et al. (2012) Immunobiology 217:521.

Customers Who Viewed This Item Also Viewed...

DCDL40
Species: Hu
Applications: ELISA
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
NBP2-25208
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
7268-CT
Species: Hu
Applications: BA
MAB140
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
NBP2-29661
Species: Hu, Mu, Rt
Applications: ELISA
6507-IL/CF
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
MAB4364
Species: Rt
Applications: ICC, IHC, IP, KO, WB
AF2849
Species: Mu, Rt
Applications: WB
NBP2-02665
Species: Ca, Hu, Pm, Po, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
DY417
Species: Mu
Applications: ELISA
NBP1-72042
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
M6000B
Species: Mu
Applications: ELISA
202-IL
Species: Hu
Applications: BA
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
AF796
Species: Mu
Applications: AdBlk, IHC, WB

Publications for CD40/TNFRSF5 (1215-CD)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Binding Assay, Bioassay, ELISA Developmet.


Filter By Application
Binding Assay
(2)
Bioassay
(1)
ELISA Developmet
(1)
All Applications
Filter By Species
Mouse
(4)
All Species

Reviews for CD40/TNFRSF5 (1215-CD) (0)

There are no reviews for CD40/TNFRSF5 (1215-CD). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CD40/TNFRSF5 (1215-CD). (Showing 1 - 2 of 2 FAQs).

  1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
    • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
  2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
    • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

Additional CD40/TNFRSF5 Products

Blogs on CD40/TNFRSF5

There are no specific blogs for CD40/TNFRSF5, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Cd40
Uniprot