Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF Summary
Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD40/TNFRSF5 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse CD40 Ligand/TNFSF5
(Catalog #
8230-CL)
that produces 50% optimal binding is found to be 100-600 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD40/TNFRSF5 protein
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
46 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
58-61 kDa, reducing conditions
Publications
Read Publications using 1215-CD in the following applications:
TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
tumor necrosis factor receptor superfamily member 5
tumor necrosis factor receptor superfamily, member 5
Background
CD40, also known as TNFRSF5, is a 32 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily. Mature mouse CD40 consists of a 174 amino acid (aa) extracellular domain, a transmembrane domain, and a 74 aa cytoplasmic domain (1, 2). The extracellular domain of mouse CD40 shares 58% and 81% aa sequence identity with human and rat CD40, respectively. Multiple mouse CD40 splice variants have been identified including soluble isoforms. CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (3, 4). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of intracellular signaling in both CD40 and CD40 Ligand expressing cells (5). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (6, 7). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (8, 9). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (10, 11). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (12, 13).
Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620.
Torres, R.M. et al. (1992) J. Immunol. 149:2641.
van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
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FAQs for CD40/TNFRSF5 (1215-CD). (Showing 1 - 2 of 2 FAQs).
I wanna know does CD40 antibody for IHC-P comes with positive control slide?
Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
Unfortunately, none of our CD40 antibodies have been specifically tested for this application.
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