Recombinant Mouse CCL21/6Ckine Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CCL21, also known as 6Ckine, TCA-4, SLC, Exodus-2, and A21, is a 12 kDa homeostatic chemokine that plays an important role in adaptive immune responses and inflammation (1). Unlike other CC chemokines, mouse CCL21 has a 36 amino acid (aa) C-terminal extension which mediates its attachment to carbohydrate structures and extracellular matrix components (2, 3). Mature mouse CCL21 shares 71% and 84% aa sequence identity with human and rat CCL21, respectively (4‑6). In mouse, three genes encode CCL21, with B and C forms being identical and the A form carrying serine at aa 65 instead of leucine. Both human and mouse CCL21 signal through the chemokine receptor CCR7, while mouse CCL21 additionally can signal through CXCR3 (7). CCL21 is constitutively presented on initial lymphatic vessels, high endothelial venules (HEV), and lymph node dendritic cells (DC) (8‑10). Immobilized CCL21 promotes the docking of DC to lymphatic vessels and the retention of T cells by lymph node DC, resulting in T cell priming for activation (8, 9). DC interaction with the anchored chemokine can induce CCL21 cleavage and release of an 8 kDa fragment that lacks the C‑terminal extension (10). During chronic inflammation or tissue damage, CCL21 is up‑regulated on local vascular endothelial cells, macrophages, T cells, and neurons (11‑14). In these settings, it promotes fibrosis, inflammatory cytokine production, and neuropathic pain (12‑14). The soluble chemokine is elevated in rheumatoid arthritis synovial fluid and in the serum of coronary artery disease patients (11, 13). CCL21 has been shown to exert either angiogenic or angiostatic effects (11, 15, 16). These effects, in combination with the ability of CCL21 to attract immune suppressor cells (Treg and MDSC) to a tumor site can have positive or negative effects on tumor progression (16, 17).

We have publications tested in 3 confirmed species: Human, Mouse, Ovine.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), In Vivo, Western Blot.

Showing Publications 1 - 10 of 52. Show All 52 Publications. Collapse Publications.

Publications using 457-6C/CF	Applications	Species
Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function PLoS ONE, 2023-01-20;18(1):e0280590. 2023-01-20 [PMID: 36662882] (Bioassay, Human)	Bioassay	Human
J Liu, Y Cheng, X Zhang, Y Chen, H Zhu, K Chen, S Liu, Z Li, X Cao Glycosyltransferase Extl1 promotes CCR7-mediated dendritic cell migration to restrain infection and autoimmunity Cell Reports, 2023-01-18;42(1):111991. 2023-01-18 [PMID: 36656709] (Bioassay, Mouse)	Bioassay	Mouse
EY Osorio, A Uscanga-Pa, GE Patterson, E Cordova, BL Travi, L Soong, PC Melby Malnutrition-related parasite dissemination from the skin in visceral leishmaniasis is driven by PGE2-mediated amplification of CCR7-related trafficking of infected inflammatory monocytes PloS Neglected Tropical Diseases, 2023-01-11;17(1):e0011040. 2023-01-11 [PMID: 36630476] (Bioassay, Mouse)	Bioassay	Mouse
EW Wolf, ZP Howard, L Duan, H Tam, Y Xu, JG Cyster GPR174 signals via Galphas to control a CD86-containing gene expression program in B cells Proceedings of the National Academy of Sciences of the United States of America, 2022-05-31;119(23):e2201794119. 2022-05-31 [PMID: 35639700] (Bioassay, Mouse)	Bioassay	Mouse
W Piao, L Li, V Saxena, J Iyyathurai, R Lakhan, Y Zhang, IT Lape, C Paluskievi, KL Hippen, Y Lee, E Silverman, MW Shirkey, LV Riella, BR Blazar, JS Bromberg PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration Nature Communications, 2022-04-21;13(1):2176. 2022-04-21 [PMID: 35449134] (Bioassay, Mouse)	Bioassay	Mouse
X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion Nature, 2021-11-03;0(0):. 2021-11-03 [PMID: 34732895] (Bioassay, Mouse)	Bioassay	Mouse
T Nakatani, K Tsujimoto, J Park, T Jo, T Kimura, Y Hayama, H Konaka, T Morita, Y Kato, M Nishide, S Koyama, S Nada, M Okada, H Takamatsu, A Kumanogoh The lysosomal Ragulator complex plays an essential role in leukocyte trafficking by activating myosin II Nature Communications, 2021-06-07;12(1):3333. 2021-06-07 [PMID: 34099704] (Bioassay, Mouse)	Bioassay	Mouse
FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense Cellular and Molecular Gastroenterology and Hepatology, 2021-03-03;0(0):. 2021-03-03 [PMID: 33676046] (Bioassay, Mouse)	Bioassay	Mouse
T Koga, F Sasaki, K Saeki, S Tsuchiya, T Okuno, M Ohba, T Ichiki, S Iwamoto, H Uzawa, K Kitajima, C Meno, E Nakamura, N Tada, Y Fukui, J Kikuta, M Ishii, Y Sugimoto, M Nakao, T Yokomizo Expression of leukotriene B4 receptor 1 defines functionally distinct DCs that control allergic skin inflammation Cell Mol Immunol, 2020-10-09;0(0):. 2020-10-09 [PMID: 33037399] (Bioassay, Mouse)	Bioassay	Mouse
J Yan, SP Pandey, BJ Barnes, JR Turner, C Abraham T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation Cell Rep, 2020-06-30;31(13):107820. 2020-06-30 [PMID: 32610123] (Bioassay, Mouse)	Bioassay	Mouse
H Zhang, Y Zheng, Y Pan, C Lin, S Wang, Z Yan, L Lu, G Ge, J Li, YA Zeng, J Chen A mutation that blocks integrin alpha4beta7 activation prevents adaptive immune-mediated colitis without increasing susceptibility to innate colitis BMC Biol., 2020-06-10;18(1):64. 2020-06-10 [PMID: 32522281] (Bioassay, Mouse)	Bioassay	Mouse
N Trempolec, C Degavre, B Doix, D Brusa, C Corbet, O Feron Acidosis-Induced TGF-beta2 Production Promotes Lipid Droplet Formation in Dendritic Cells and Alters Their Potential to Support Anti-Mesothelioma T Cell Response Cancers (Basel), 2020-05-19;12(5):. 2020-05-19 [PMID: 32438640] (Bioassay, Mouse)	Bioassay	Mouse
N Trempolec, B Doix, C Degavre, D Brusa, C Bouzin, O Riant, O Feron Photodynamic Therapy-Based Dendritic Cell Vaccination Suited to Treat Peritoneal Mesothelioma Cancers (Basel), 2020-02-27;12(3):. 2020-02-27 [PMID: 32120810] (Bioassay, Mouse)	Bioassay	Mouse
L Li, MW Shirkey, T Zhang, Y Xiong, W Piao, V Saxena, C Paluskievi, YS Lee, N Toney, BM Cerel, Q Li, T Simon, KD Smith, KL Hippen, BR Blazar, R Abdi, JS Bromberg The lymph node stromal laminin alpha 5 shapes alloimmunity J. Clin. Invest., 2020-05-01;0(0):. 2020-05-01 [PMID: 32017712] (Bioassay, Mouse)	Bioassay	Mouse
S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 2019-06-25;10(0):1421. 2019-06-25 [PMID: 31293551] (Binding Assay, Mouse)	Binding Assay	Mouse
ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling J. Clin. Invest., 2019-05-20;130(0):2555-2570. 2019-05-20 [PMID: 31107242] (Bioassay, Mouse)	Bioassay	Mouse
L Simula, I Pacella, A Colamatteo, C Procaccini, V Cancila, M Bordi, C Tregnago, M Corrado, M Pigazzi, V Barnaba, C Tripodo, G Matarese, S Piconese, S Campello Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming Cell Rep, 2018-12-11;25(11):3059-3073.e10. 2018-12-11 [PMID: 30540939] (Bioassay, Mouse)	Bioassay	Mouse
FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus Elife, 2018-09-06;7(0):. 2018-09-06 [PMID: 30187863] (Mouse)		Mouse
W Piao, Y Xiong, K Famulski, CC Brinkman, L Li, N Toney, C Wagner, V Saxena, T Simon, JS Bromberg Regulation of T cell afferent lymphatic migration by targeting LT?R-mediated non-classical NF?B signaling Nat Commun, 2018-08-01;9(1):3020. 2018-08-01 [PMID: 30069025] (Bioassay, Mouse)	Bioassay	Mouse
R Mashud, A Nomachi, A Hayakawa, K Kubouchi, S Danno, T Hirata, K Matsuo, T Nakayama, R Satoh, R Sugiura, M Abe, K Sakimura, S Wakana, H Ohsaki, S Kamoshida, H Mukai Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1 Sci Rep, 2017-08-09;7(1):7663. 2017-08-09 [PMID: 28794483] (Bioassay, Mouse)	Bioassay	Mouse
M Burbage, SJ Keppler, B Montaner, PK Mattila, FD Batista The Small Rho GTPase TC10 Modulates B Cell Immune Responses J. Immunol., 2017-07-26;0(0):. 2017-07-26 [PMID: 28747344] (Bioassay, Mouse)	Bioassay	Mouse
T Saito, M Yano, Y Ohki, M Tomura, N Nakano Occludin Expression in Epidermal ?? T Cells in Response to Epidermal Stress Causes Them To Migrate into Draining Lymph Nodes J. Immunol., 2017-05-31;0(0):. 2017-05-31 [PMID: 28566372] (Bioassay, Mouse)	Bioassay	Mouse
A Shiraishi, T Uruno, F Sanematsu, M Ushijima, D Sakata, T Hara, Y Fukui DOCK8 Protein Regulates Macrophage Migration through Cdc42 Protein Activation and LRAP35a Protein Interaction J. Biol. Chem, 2016-12-27;292(6):2191-2202. 2016-12-27 [PMID: 28028174] (Bioassay, Mouse)	Bioassay	Mouse
Istvan Szatmari Immunogenic Dendritic Cell Generation from Pluripotent Stem Cells by Ectopic Expression of Runx3 J. Immunol., 2016-11-16;0(0):. 2016-11-16 [PMID: 27852743] (Bioassay, Mouse)	Bioassay	Mouse
Efficient Plasma Cell Differentiation and Trafficking Require Cxcr4 Desensitization Cell Rep, 2016-09-27;17(1):193-205. 2016-09-27 [PMID: 27681431] (Bioassay, Mouse)	Bioassay	Mouse
CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima Nat Immunol, 2016-09-26;0(0):. 2016-09-26 [PMID: 27668800] (In Vivo, Mouse)	In Vivo	Mouse
ADAP plays a pivotal role in CD4+ T cell activation but is only marginally involved in CD8+ T cell activation, differentiation, and immunity to pathogens J Leukoc Biol, 2016-09-07;0(0):. 2016-09-07 [PMID: 27605210] (Bioassay, Mouse)	Bioassay	Mouse
WNK1 kinase balances T cell adhesion versus migration in vivo Nat Immunol, 2016-07-11;0(0):. 2016-07-11 [PMID: 27400149] (Bioassay, Human)	Bioassay	Human
Tsun-Ho Lo Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration J Immunol, 2016-06-17;0(0):. 2016-06-17 [PMID: 27316686] (Bioassay, Mouse)	Bioassay	Mouse
Gomez D, Diehl M, Crosby E, Weinkopff T, Debes G Effector T Cell Egress via Afferent Lymph Modulates Local Tissue Inflammation. J Immunol, 2015-09-09;195(8):3531-6. 2015-09-09 [PMID: 26355150] (Bioassay, Mouse)	Bioassay	Mouse
Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander J, Nelson C, Jakobsson L, Betsholtz C, Sund M, Karlsson M, Fuxe J TGF-beta1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis. Oncogene, 2015-05-11;35(6):748-60. 2015-05-11 [PMID: 25961925] (Bioassay, Mouse)	Bioassay	Mouse
Badr G, Sayed A, Abdel-Maksoud M, Mohamed A, El-Amir A, Abdel-Ghaffar F, Al-Quraishy S, Mahmoud M Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFkappaB and ERK. PLoS ONE, 2015-04-24;10(4):e0125340. 2015-04-24 [PMID: 25909640] (Bioassay, Mouse)	Bioassay	Mouse
Ballesteros A, Mentink-Kane M, Warren J, Kaplan G, Dveksler G Induction and activation of latent transforming growth factor-beta1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1). J Biol Chem, 2014-12-29;290(7):4422-31. 2014-12-29 [PMID: 25548275] (Bioassay, Mouse)	Bioassay	Mouse
Nakai A, Hayano Y, Furuta F, Noda M, Suzuki K Control of lymphocyte egress from lymph nodes through beta2-adrenergic receptors. J Exp Med, 2014-11-24;211(13):2583-98. 2014-11-24 [PMID: 25422496] (Bioassay, Mouse)	Bioassay	Mouse
Knowlden S, Capece T, Popovic M, Chapman T, Rezaee F, Kim M, Georas S Regulation of T cell motility in vitro and in vivo by LPA and LPA2. PLoS ONE, 2014-07-08;9(7):e101655. 2014-07-08 [PMID: 25003200] (Bioassay, Mouse)	Bioassay	Mouse
Katakai T, Kondo N, Ueda Y, Kinashi T Autotaxin produced by stromal cells promotes LFA-1-independent and Rho-dependent interstitial T cell motility in the lymph node paracortex. J Immunol, 2014-06-16;193(2):617-26. 2014-06-16 [PMID: 24935929] (Bioassay, Mouse)	Bioassay	Mouse
Geherin , Skye A, Wilson , R Paul, Jennrich , Silke, Debes , Gudrun F CXCR4 is dispensable for T cell egress from chronically inflamed skin via the afferent lymph. PLoS ONE, 2014-04-21;9(4):e95626. 2014-04-21 [PMID: 24752354] (Bioassay, Mouse, Ovine)	Bioassay	Mouse, Ovine
Xu Y, Pektor S, Balkow S, Hemkemeyer S, Liu Z, Grobe K, Hanley P, Shen L, Bros M, Schmidt T, Bahler M, Grabbe S Dendritic cell motility and T cell activation requires regulation of Rho-cofilin signaling by the Rho-GTPase activating protein myosin IXb. J Immunol, 2014-03-19;192(8):3559-68. 2014-03-19 [PMID: 24646736] (Bioassay, Mouse)	Bioassay	Mouse
Willinger T, Ferguson S, Pereira J, De Camilli P, Flavell R Dynamin 2-dependent endocytosis is required for sustained S1PR1 signaling. J Exp Med, 2014-03-17;211(4):685-700. 2014-03-17 [PMID: 24638168] (Bioassay, Mouse)	Bioassay	Mouse
Nomachi , Akira, Yoshinaga , Masanori, Liu , Jaron, Kanchanawong , Pakorn, Tohyama , Kiyoshi, Thumkeo , Dean, Watanabe , Takeshi, Narumiya , Shuh, Hirata , Takako Moesin controls clathrin-mediated S1PR1 internalization in T cells. PLoS ONE, 2013-12-16;8(12):e82590. 2013-12-16 [PMID: 24358210] (Bioassay, Mouse)	Bioassay	Mouse
Luckashenak N, Wahe A, Breit K, Brakebusch C, Brocker T Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol, 2012-12-03;190(1):27-35. 2012-12-03 [PMID: 23209325] (Cell Culture, Mouse)	Cell Culture	Mouse
Hyun YM, Sumagin R, Sarangi PP Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels. J. Exp. Med., 2012-06-18;209(7):1349-62. 2012-06-18 [PMID: 22711877] (Bioassay, Mouse)	Bioassay	Mouse
Ippagunta SK, Malireddi RK, Shaw PJ The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization. Nat. Immunol., 2011-09-04;12(10):1010-6. 2011-09-04 [PMID: 21892172] (Bioassay, Mouse)	Bioassay	Mouse
Rehm A, Mensen A, Schradi K, Gerlach K, Wittstock S, Winter S, Buchner G, Dorken B, Lipp M, Hopken UE Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid organs. Blood, 2011-05-17;118(4):1020-33. 2011-05-17 [PMID: 21586747] (Bioassay, Mouse)	Bioassay	Mouse
Biber K, Tsuda M, Tozaki-Saitoh H Neuronal CCL21 up-regulates microglia P2X4 expression and initiates neuropathic pain development. EMBO J., 2011-03-25;30(9):1864-73. 2011-03-25 [PMID: 21441897] (In Vivo, Mouse)	In Vivo	Mouse
Marttila-Ichihara F, Turja R, Miiluniemi M, Karikoski M, Maksimow M, Niemela J, Martinez-Pomares L, Salmi M, Jalkanen S Macrophage mannose receptor on lymphatics controls cell trafficking. Blood, 2008-04-23;112(1):64-72. 2008-04-23 [PMID: 18434610] (Bioassay, Mouse)	Bioassay	Mouse
Pham TH, Okada T, Matloubian M, Lo CG, Cyster JG S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress. Immunity, 2007-12-27;28(1):122-33. 2007-12-27 [PMID: 18164221] (Bioassay, Mouse)	Bioassay	Mouse
Kabashima K, Shiraishi N, Sugita K, Mori T, Onoue A, Kobayashi M, Sakabe J, Yoshiki R, Tamamura H, Fujii N, Inaba K, Tokura Y CXCL12-CXCR4 engagement is required for migration of cutaneous dendritic cells. Am. J. Pathol., 2007-09-06;171(4):1249-57. 2007-09-06 [PMID: 17823289] (Bioassay, Mouse)	Bioassay	Mouse
Fujita H, Asahina A, Komine M, Tamaki K The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Cell. Immunol., 2007-05-15;245(2):70-9. 2007-05-15 [PMID: 17507001] (Bioassay, Mouse)	Bioassay	Mouse
Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 2004-09-09;105(1):31-9. 2004-09-09 [PMID: 15358618] (Bioassay, Mouse)	Bioassay	Mouse
Flanagan K, Glover RT, Horig H, Yang W, Kaufman HL Local delivery of recombinant vaccinia virus expressing secondary lymphoid chemokine (SLC) results in a CD4 T-cell dependent antitumor response. Vaccine, 2004-07-29;22(21):2894-903. 2004-07-29 [PMID: 15246626] (Western Blot)	Western Blot
Tolba KA, D†­ÃÅê†Ò, Bowers WJ, Muller J, Housekneckt V, Giuliano RE, Federoff HJ, Rosenblatt JD Herpes simplex virus (HSV) amplicon-mediated codelivery of secondary lymphoid tissue chemokine and CD40L results in augmented antitumor activity. Îζ)Š4wJ]¾b.ŠKd•ÿ¶fäMò&¨k³, 2002-11-15;62(22):6545-51. 2002-11-15 [PMID: 12438249] (Bioassay, ELISA (Standard), Human)	Bioassay, ELISA (Standard)	Human
Show All 52 Publications. Collapse Publications.

Array 457-6C/CF

• CCL21/6Ckine Antibodies
• CCL21/6Ckine Lysate
• CCL21/6Ckine Proteins
• CCL21/6Ckine ELISAs
• CCL21/6Ckine Proteins and Enzymes