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Recombinant Human TNF-alpha Protein, CF

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Recombinant Human TNF-alpha (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
Recombinant Human TNF-alpha (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
210-TA-005
Carrier Free
210-TA-02M/CF
Carrier Free
210-TA-100/CF
Carrier Free
210-TA-005/CF
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Recombinant Human TNF-alpha Protein, CF Summary

Additional Information
A new economically priced TNF-alpha is available! The new protein is HEK293 expressed!
Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
Source
E. coli-derived human TNF-alpha protein
Val77-Leu233, with and without an N-terminal Met
Accession #
N-terminal Sequence
Met & Val77
Protein/Peptide Type
Recombinant Proteins
Gene
TNF
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
210-TA/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (5 & 20 µg) is supplied as a 0.2 µm filtered solution in PBS. 
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 0.1-1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TNF-alpha Protein, CF

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha . TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Pennica, D. et al. (1984) Nature 312:724.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.

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Recombinant Human
TNF-alpha Protein, CF
210-TA/CF
Recombinant Human TNF-alpha Protein, CF
Species: Hu
Applications: Bioactivity

Publications for TNF-alpha (210-TA/CF)(773)

We have publications tested in 17 confirmed species: Human, Mouse, Rat, Bovine, Canine, Complex Species Category, Drosophila, Feline, Guinea Pig, Hamster, N/A, Ovine, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 14 applications: Bioassay, Cell Culture, ELISA (Capture), ELISA (Standard), ELISA Control, ELISA Standard, Hemofiltration Procedure, IHC-P, In Vivo, SDS-Page, Spheroid Culture, Stimulation, Surface Plasmon Resonance (SPR, Western Blot.


Filter By Application
Bioassay
(693)
Cell Culture
(42)
ELISA (Capture)
(1)
ELISA (Standard)
(15)
ELISA Control
(1)
ELISA Standard
(2)
Hemofiltration Procedure
(1)
IHC-P
(1)
In Vivo
(6)
SDS-Page
(1)
Spheroid Culture
(1)
Stimulation
(3)
Surface Plasmon Resonance (SPR
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(695)
Mouse
(36)
Rat
(10)
Bovine
(6)
Canine
(2)
Complex Species Category
(2)
Drosophila
(1)
Feline
(1)
Guinea Pig
(1)
Hamster
(1)
N/A
(1)
Ovine
(1)
Porcine
(4)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 773. Show All 773 Publications.
Publications using 210-TA/CF Applications Species
Ma, HY;Li, Q;Wong, WR;N'Diaye, EN;Caplazi, P;Bender, H;Huang, Z;Arlantico, A;Jeet, S;Wong, A;Emson, C;Brightbill, H;Tam, L;Newman, R;Roose-Girma, M;Sandoval, W;Ding, N; LOXL4, but not LOXL2, is the critical determinant of pathological collagen cross-linking and fibrosis in the lung Science advances 2023-05-26 [PMID: 37235663] (Bioassay, Human) Bioassay Human
Cheng, RYS;Ridnour, LA;Wink, AL;Gonzalez, AL;Femino, EL;Rittscher, H;Somasundaram, V;Heinz, WF;Coutinho, L;Rangel, MC;Edmondson, EF;Butcher, D;Kinders, RJ;Li, X;Wong, STC;McVicar, DW;Anderson, SK;Pore, M;Hewitt, SM;Billiar, TR;Glynn, SA;Chang, JC;Lockett, SJ;Ambs, S;Wink, DA; Interferon-gamma is quintessential for NOS2 and COX2 expression in ER- breast tumors that lead to poor outcome Cell death & disease 2023-05-11 [PMID: 37169743] (Bioassay, Human) Bioassay Human
P Dörschmann, C Seeba, T Thalenhors, J Roider, A Klettner Anti-inflammatory properties of antiangiogenic fucoidan in retinal pigment epithelium cells Heliyon, 2023;9(4):e15202. 2023 [PMID: 37123974] (Cell Culture, Porcine) Cell Culture Porcine
P Dörschmann, C Seeba, T Thalenhors, J Roider, A Klettner Anti-inflammatory properties of antiangiogenic fucoidan in retinal pigment epithelium cells Heliyon, 2023-04-10;9(4):e15202. 2023-04-10 [PMID: 37123974] (Cell Culture, Porcine) Cell Culture Porcine
IN Boys, AG Johnson, M Quinlan, PJ Kranzusch, NC Elde Structural homology screens reveal poxvirus-encoded proteins impacting inflammasome-mediated defenses bioRxiv : the preprint server for biology, 2023-04-03;0(0):. 2023-04-03 [PMID: 36909515] (Bioassay, Human) Bioassay Human
Y Nie, L Mou, Q Long, D Deng, R Hu, J Cheng, J Wu SARS-CoV-2 ORF3a positively regulates NF-kappaB activity by enhancing IKKbeta-NEMO interaction Virus research, 2023-03-13;328(0):199086. 2023-03-13 [PMID: 36894068] (Bioassay, Human) Bioassay Human
T Zyrianova, K Zou, B Lopez, A Liao, C Gu, R Olcese, A Schwingsha Activation of Endothelial Large Conductance Potassium Channels Protects against TNF-alpha-Induced Inflammation International Journal of Molecular Sciences, 2023-02-17;24(4):. 2023-02-17 [PMID: 36835507] (Bioassay, Human) Bioassay Human
F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells bioRxiv : the preprint server for biology, 2023-02-13;0(0):. 2023-02-13 [PMID: 36789428] (Bioassay, Human) Bioassay Human
DI Cho, MJ Ahn, HH Cho, M Cho, JH Jun, BG Kang, SY Lim, SJ Yoo, MR Kim, HS Kim, SJ Lee, LT Dat, C Lee, YS Kim, Y Ahn ANGPTL4 stabilizes atherosclerotic plaques and modulates the phenotypic transition of vascular smooth muscle cells through KLF4 downregulation Experimental & Molecular Medicine, 2023-02-13;0(0):. 2023-02-13 [PMID: 36782020] (Bioassay, Human) Bioassay Human
RD de Ruiter, LE Wisse, T Schoenmake, M Yaqub, G Sánchez-Du, EMW Eekhoff, D Micha TGF-Beta Induces Activin A Production in Dermal Fibroblasts Derived from Patients with Fibrodysplasia Ossificans Progressiva International Journal of Molecular Sciences, 2023-01-24;24(3):. 2023-01-24 [PMID: 36768622] (Bioassay, Human) Bioassay Human
Show All 773 Publications.

Reviews for TNF-alpha (210-TA/CF) (0)

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FAQs for TNF-alpha (210-TA/CF). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Bioinformatics

Gene Symbol TNF
Uniprot