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Recombinant Human Pro-Collagen III alpha 1/COL3A1, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human Pro-Collagen III alpha 1/COL3A1, CF Summary

Details of Functionality
Measured by the cleavage of its C-terminal propeptide by Recombinant Human BMP‑1/PCP (Catalog # 1927-ZN). >50% of full-length Pro-Collagen III alpha 1 is cleaved by recombinant human BMP-1, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human Collagen III alpha 1/COL3A1 protein
Thr1097-Leu1466, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
His
Protein/Peptide Type
Recombinant Enzymes
Gene
COL3A1
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
41-46 kDa, reducing conditions
Publications
Read Publications using
7294-CL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Assay Procedure
  • Assay Buffer: 25 mM HEPES, 0.01% Brij, pH 7.5
  • Recombinant Human Pro Collagen III  alpha 1/COL3A1 (rhPro-COL3A1) (Catalog # 7294-CL)
  • Recombinant Human BMP‑1/PCP (rhBMP-1) (Catalog # 1927-ZN)
  • Reducing SDS-PAGE Sample Buffer
  • SDS-PAGE or Western Blot
  1. Dilute rhPro-COL3A1 to 75 μg/mL in Assay Buffer.
  2. Dilute rhBMP-1 to 5 μg/mL in Assay Buffer.
  3. Combine one volume of diluted rhPro-COL3A1 with three volumes of diluted rhBMP-1. For controls, combine one volume of rhPro‑COL3A1 with three volumes of Assay Buffer, as well as three volumes of rhBMP-1 with one volume of Assay Buffer.
  4. Incubate reaction vials and controls at 37 °C for one hour.
  5. After incubation, combine rhPro-COL3A1/rhBMP-1 reaction mixtures and controls with reducing SDS-PAGE gel buffer at a 2:1 (reaction mixture:gel buffer) ratio (v/v). Mix and incubate samples at 95-100 °C for 3-5 minutes to stop reactions.
  6. Load 40 μL (0.5 μg of rhPro-COL3A1) per lane and analyze the cleavage by SDS-PAGE followed by protein staining and/or Western blot.
  7. Activity calculation:
     % Cleavage = [1  -   % full-length rhPro-COL3A1 (reaction) ] x 100%
% full-length rhPro-COL3A1 (control)
Per Lane:
  • rhCOL3A1: 0.5 µg
  • rhBMP-1: 0.1 µg

Notes

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Pro-Collagen III alpha 1/COL3A1, CF

  • alpha1 (III) collagen
  • alpha-1 type III collagen
  • COL3A1
  • Collagen 3
  • collagen alpha-1(III) chain
  • Collagen III alpha 1
  • collagen, fetal
  • collagen, type III, alpha 1
  • Collagen-3
  • collagenIII
  • Collagen-III
  • EDS4A
  • EDSVASC
  • Ehlers-Danlos syndrome type IV, autosomal dominant
  • FLJ34534
  • PMGEDSV

Background

Collagen type III is found in most soft connective tissues, such as skin, lung, and the vascular system, often in association with type I collagen. It is a trimer of
alpha 1(III) chains that are linked by interchain disulfide bonds. Defective collagen type III is a cause of Ehlers-Danlos syndrome types III and IV and arterial aneurisms (1). This recombinant mini pro-alpha 1(III) collagen consists of a triple-helical region of Gly-Xaa-Yaa repeats, a short non-helical region, and the C‑terminal propeptide. The C‑terminal propeptide can be removed by the procollagen C‑proteinase (BMP-1).
  1. Kuivaniemi H. et al. (1997) Hum. Mutat. 9:300.

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Publications for Collagen III alpha 1/COL3A1 (7294-CL)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.


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(2)
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Bioinformatics

Gene Symbol COL3A1
Uniprot