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Recombinant Human IGFBP-3 Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human IGFBP-3 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.05-0.15 µg/mL in the presence of 14 ng/mL Recombinant Human IGF‑II (Catalog # 292-G2).
Source
Mouse myeloma cell line, NS0-derived human IGFBP-3 protein
Gly28-Lys291, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IGFBP3
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
37-47 kDa, reducing conditions
Publications
Read Publications using
675-B3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGFBP-3 Protein, CF

  • acid stable subunit of the 140 K IGF complex
  • binding protein 29
  • binding protein 53
  • growth hormone-dependent binding protein
  • IBP-3
  • IBP3BP-53
  • IGF-binding protein 3
  • IGFBP3
  • IGFBP-3
  • insulin-like growth factor binding protein 3
  • insulin-like growth factor-binding protein 3

Background

Insulin-like growth factor binding protein-3 (IGFBP-3) is one of six members of the insulin­like growth factor (IGF) binding protein superfamily which function to modulate the biological activity of IGF (1). Human IGFBP-3 is the major binding protein of IGF where it exists in circulation as a ternary complex with the acid-labile subunit (ALS) (2). Like other IGFBP members, human IGFBP-3 includes a cysteine-rich C-terminal domain, a highly variable central linker domain, and another
N-terminal cysteine-­rich domain (2, 3). Human IGFBP­-3 cDNA encodes a 291 amino acid (aa) precursor protein with a 27 aa signal peptide that is processed to generate the 264 aa mature protein. Mature human IGFBP-3 shares 82% aa sequence identity with both mouse and rat IGFBP-3. Post-­translational glycosylation and phosphorylation of IGFBP-3 modifies the affinities of the binding protein. Proteolysis of IGFBP-3 by tissue plasminogen activator (tPA), a disintegrin and metaloproteases (ADAMs), and prostate specific antigen (PSA) contributes to IGFBP-3 degradation or a reduction in its affinity for IGF (4-6). The majority of soluble IGFBP-3 found in circulation is secreted from hepatic non-parenchymal cells. IGFBP-3 expression can be modulated by p53 as well as by various cytokines and growth factors (7, 8). In addition to its role in stabilizing and transporting circulating IGF, IGFBP-3 has been shown to potentiate EGF-EGFR-mediated cell growth through the activation of sphingosine kinase1 (SPHK1) and sphingosin-1-phosphate (S1P) (9, 10). IGFBP-3 has also been shown to modulate adipogenesis (11). Binding of IGFBP-3 to non-IGF-related ligands has been shown to regulate TGF-beta signaling, DNA damage, apoptosis, autophagy, and gene transcription (12). Interactions with non-IGF-related ligands is thought to contribute, in part, to the dichotomous stimulatory and inhibitory effects of IGFBP-3 on cell growth (2).

  1. Shimasaki, S. and N. Ling (1991) Prog. Growth Factor Res. 3:243.
  2. Baxter, R.C. (2013) J. Cell Commun. Signal 7:179.
  3. Baxter, R.C. (2014) Nat. Rev. Cancer 14:329.
  4. Mochizuki, S. et al. (2004) Biochem. Biophys. Res. Commun. 315:79.
  5. Cohen, P. et al. (1994) J. Endocrinol. 142:407.
  6. Bang, P. (1995) Prog. Growth Factor Res. 6:285.
  7. Perks, C.M. and J.M. Holly (2008) J. Mammary Gland Biol. Neoplasia 13:455.
  8. Chan, K. and E.M. Spencer (1997) Endocrine 7:95.
  9. Guix, M. et al. (2008) J. Clin. Invest. 118:2609.
  10. Martin, J.L. et al. (2009) J. Biol. Chem. 284:25542.
  11. Chan, S.S. et al. (2009) Am. J. Physiol. Endocrinol. Metab. 296:E654.
  12. Martin, J.L. and R.C. Baxter (2011) Growth Factors 29:235.

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Recombinant Human
IGFBP-3 Protein, CF
675-B3
Recombinant Human IGFBP-3 Protein, CF
Species: Hu
Applications: Bioactivity

Publications for IGFBP-3 (675-B3)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Enzyme Assay, Western Blot.


Filter By Application
Bioassay
(10)
Enzyme Assay
(2)
Western Blot
(1)
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Filter By Species
Human
(11)
Mouse
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 675-B3 Applications Species
EFY Ng, A Kaida, H Nojima, M Miura Roles of IGFBP-3 in cell migration and growth in an endophytic tongue squamous cell carcinoma cell line Scientific Reports, 2022-07-07;12(1):11503. 2022-07-07 [PMID: 35798794] (Bioassay, Human) Bioassay Human
HJ Shih, HF Chang, CL Chen, PL Torng Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer Scientific Reports, 2021-11-25;11(1):22925. 2021-11-25 [PMID: 34824343] (Bioassay, Human) Bioassay Human
E Wirthgen, S Goumon, M Kunze, C Walz, M Spitschak, A Tuchschere, J Brown, C Höflich, L Faucitano, A Hoeflich Effects of Transport Duration and Environmental Conditions in Winter or Summer on the Concentrations of Insulin-Like Growth Factors and Insulin-Like Growth Factor-Binding Proteins in the Plasma of Market-Weight Pigs Front Endocrinol (Lausanne), 2018-02-13;9(0):36. 2018-02-13 [PMID: 29487569] (Western Blot, Human) Western Blot Human
Matthew N Svalina IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma Sci Rep, 2016-06-03;6(0):27012. 2016-06-03 [PMID: 27255663] (Bioassay, Human) Bioassay Human
Elzi , David J, Lai , Yanlai, Song , Meihua, Hakala , Kevin, Weintraub , Susan T, Shiio , Yuzuru Plasminogen activator inhibitor 1--insulin-like growth factor binding protein 3 cascade regulates stress-induced senescence. Proc Natl Acad Sci U S A, 2012-07-09;109(30):12052-7. 2012-07-09 [PMID: 22778398] (Bioassay, Human) Bioassay Human
Bogatkevich GS, Ludwicka-Bradley A, Highland KB, Hant F, Nietert PJ, Singleton CB, Feghali-Bostwick CA, Silver RM Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis. Arthritis Rheum., 2007-07-01;56(7):2432-42. 2007-07-01 [PMID: 17599773] (Bioassay, Human) Bioassay Human
Rosendahl AH, Forsberg G IGF-I and IGFBP-3 augment transforming growth factor-beta actions in human renal carcinoma cells. Kidney Int., 2006-09-13;70(9):1584-90. 2006-09-13 [PMID: 16969385] (Bioassay, Human) Bioassay Human
Schnapp LM, Donohoe S, Chen J, Sunde DA, Kelly PM, Ruzinski J, Martin T, Goodlett DR Mining the acute respiratory distress syndrome proteome: identification of the insulin-like growth factor (IGF)/IGF-binding protein-3 pathway in acute lung injury. Am. J. Pathol., 2006-07-01;169(1):86-95. 2006-07-01 [PMID: 16816363] (Bioassay, Human) Bioassay Human
Cortesio CL, Jiang W Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch. Biochem. Biophys., 2006-03-03;449(1):164-70. 2006-03-03 [PMID: 16554018] (Enzyme Assay, Human) Enzyme Assay Human
Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability. Biochem. Biophys. Res. Commun., 2005-08-05;333(3):1011-6. 2005-08-05 [PMID: 15964556] (Bioassay, Enzyme Assay, Human) Bioassay, Enzyme Assay Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol IGFBP3
Uniprot