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Recombinant Human CX3CL1/Fractalkine (Chemokine Domain)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
362-CX-025/CF
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Recombinant Human CX3CL1/Fractalkine (Chemokine Domain) Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED50 for this effect is 2‑10 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CX3CR1. The ED50 for this effect is 0.3-1.5 ng/mL.
Source
E. coli-derived human CX3CL1/Fractalkine protein
Gln25-Gly100
Accession #
N-terminal Sequence
Gln25
Protein/Peptide Type
Recombinant Proteins
Gene
CX3CL1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
8.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
362-CX in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CX3CL1/Fractalkine (Chemokine Domain)

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes. The gene for human CX3CL1 has been mapped to chromosome 16q.

  1. Pan, Y. et al. (1997) Nature 387:611.
  2. Bazan, J.F. et al. (1997) Nature 385:640.
  3. Mackay, C.R. (1997) Current Biology 7:R384.

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Recombinant Human
CX3CL1/Fractalkine (C ...
362-CX
Recombinant Human CX3CL1/Fractalkine (Chemokine Domain)
Species: Hu
Applications: Bioactivity

Publications for CX3CL1/Fractalkine (362-CX)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Antibody Absorption, Bioassay, Control, In Vivo, Western Blot.


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Antibody Absorption
(1)
Bioassay
(5)
Control
(1)
In Vivo
(2)
Western Blot
(1)
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Human
(5)
Mouse
(2)
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Showing Publications 1 - 9 of 9.
Publications using 362-CX Applications Species
AL Collar, M Swamydas, M O'Hayre, MS Sajib, KW Hoffman, SP Singh, A Mourad, MD Johnson, EM Ferre, JM Farber, JK Lim, CM Mikelis, JS Gutkind, MS Lionakis The homozygous CX3CR1-M280 mutation impairs human monocyte survival JCI Insight, 2018-02-08;3(3):. 2018-02-08 [PMID: 29415879] (Bioassay, Human) Bioassay Human
Y Park, J Lee, JY Kwak, K Noh, E Yim, HK Kim, YJ Kim, HE Broxmeyer, JA Kim Fractalkine induces angiogenic potential in CX3CR1-expressing monocytes J. Leukoc. Biol., 2017-12-28;0(0):. 2017-12-28 [PMID: 28978662] (Bioassay, Mouse) Bioassay Mouse
Plasmodium falciparum proteins involved in cytoadherence of infected erythrocytes to chemokine CX3CL1 Sci Rep, 2016-09-22;6(0):33786. 2016-09-22 [PMID: 27653778] (Bioassay, Human) Bioassay Human
Truman LA, Ford CA, Pasikowska M, Pound JD, Wilkinson SJ, Dumitriu IE, Melville L, Melrose LA, Ogden CA, Nibbs R, Graham G, Combadiere C, Gregory CD CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis. Blood, 2008-09-17;112(13):5026-36. 2008-09-17 [PMID: 18799722] (Bioassay, Human) Bioassay Human
Holmes FE, Arnott N, Vanderplank P, Kerr NC, Longbrake EE, Popovich PG, Imai T, Combadiere C, Murphy PM, Wynick D Intra-neural administration of fractalkine attenuates neuropathic pain-related behaviour. J. Neurochem., 2008-04-12;106(2):640-9. 2008-04-12 [PMID: 18410510] (In Vivo, Mouse) In Vivo Mouse
Clark AK, Yip PK, Grist J, Gentry C, Staniland AA, Marchand F, Dehvari M, Wotherspoon G, Winter J, Ullah J, Bevan S, Malcangio M Inhibition of spinal microglial cathepsin S for the reversal of neuropathic pain. Proc. Natl. Acad. Sci. U.S.A., 2007-06-05;104(25):10655-60. 2007-06-05 [PMID: 17551020] (In Vivo, Rat) In Vivo Rat
Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation. Eur. J. Neurosci., 2004-12-01;20(12):3222-32. 2004-12-01 [PMID: 15610155] (Bioassay, Human) Bioassay Human
Latchney LR, Fallon MA, Culp DJ, Gelbard HA, Dewhurst S Immunohistochemical assessment of fractalkine, inflammatory cells, and human herpesvirus 7 in human salivary glands. J. Histochem. Cytochem., 2004-05-01;52(5):671-81. 2004-05-01 [PMID: 15100244] (Control, Human) Control Human
Lucas AD, Chadwick N, Warren BF, Jewell DP, Gordon S, Powrie F, Greaves DR The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo. Am. J. Pathol., 2001-03-01;158(3):855-66. 2001-03-01 [PMID: 11238035] (Antibody Absorption, Western Blot) Antibody Absorption, Western Blot

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Recombinant Human CX3CL1/Fractalkine (Catalog&nbsp;# 365-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with&nbsp;mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog&nbsp;# AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100&nbsp;ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;# AF365). The ND50 is typically 0.75-4.5&nbsp;µg/mL.
CX3CL1/Fractalkine Antibody [Unconjugated]
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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
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Bioinformatics

Gene Symbol CX3CL1
Uniprot