Reactivity | HuSpecies Glossary |
Applications | Bioactivity |
Format | Carrier-Free |
Details of Functionality | Measured by its binding ability in a functional ELISA. When Recombinant Human CD90/Thy-1 His-tag is immobilized at 5.00 µg/mL (100 µL/well), Recombinant Human Galectin-1
(Catalog #
1152-GA)
binds with an ED50 of 0.400-2.00 μg/mL. |
Source | Chinese Hamster Ovary cell line, CHO-derived human CD90/Thy1 protein Gln20-Ser141, with a C-terminal 6-His tag. Accession # P04216.2 |
Accession # | |
N-terminal Sequence | Gln20 inferred from enzymatic pyroglutamate treatment revealing Lys21. |
Protein/Peptide Type | Recombinant Proteins |
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
Dilutions |
|
Theoretical MW | 14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE | 19-33 kDa, under reducing conditions. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions | Reconstitute at 500 μg/mL in PBS. |
CD90 (Cluster of differentiation 90) CD90 is a 25-35kD glycosylphosphatidylinositol (GPI)-linked glycoprotein receptor of the immunoglobulin (Ig) superfamily. It is found on murine T-cells, thymocytes, neuronal cells, granulocytic lineage-derived cells, hematopoietic stem cells, fibrobla... Read full blog post. |
How New Oval Stem Cell Marker Antibodies Will Benefit Hepatic Research A large number of antibody suppliers supply conjugated and non-conjugated marker antibodies, targeted at specific stem cell populations. Until recently the number of oval stem cell markers was extremely limited. However, we at Novus Biologicals have s... Read full blog post. |
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