Home » CCL21/6Ckine » CCL21 Peptides and Proteins » Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera), CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera), CF

 Submit a Review

Images

 
There are currently no images for CCL21/6Ckine (966-6C/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
966-6C-025
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera), CF Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.5‑1.5 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED50 for this effect is 25-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human CCL21/6Ckine protein
Human 6Ckine
(Ser24 - Pro134)
(Pro41 Ser)
Accession # NP_002980		 GS Human Fractalkine mucin-like stalk
(Phe103 - Gln341)
Accession # P78423		 KQN 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL21
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
38.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
95-105 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera), CF

  • 6Ckine
  • 6CkineSmall-inducible cytokine A21
  • Beta-chemokine exodus-2
  • CCL21
  • chemokine (C-C motif) ligand 21
  • CKb9
  • exodus-2
  • member 21
  • SCYA21
  • SCYA21MGC34555
  • secondary lymphoid tissue chemokine
  • SLC
  • SLCSecondary lymphoid-tissue chemokine
  • TCA-4

Background

Fractalkine (also known as neurotactin and CX3CL1) is one of two known transmembrane chemokines and is the only member of the CX3C chemokine subfamily. Fractalkine is a type I membrane protein containing a chemokine domain tethered on a long mucin-like stalk that is connected to a transmembrane domain and a C-terminal intracellular domain. Expression of Fractalkine has been detected on endothelial cells, neurons, dendritic cells and a subset of mononuclear phagocytes. The expression of Fractalkine is upregulated in response to inflammatory signals. Fractalkine binds with high affinity to CX3CR1 and mediates the tight binding of leukocytes expressing CX3CR1 independently of G protein activation under flow conditions. In the 6Ckine/mucin-like stalk chimera, the chemokine domain of human Fractalkine is replaced by human 6Ckine (CCL21), a ligand for CCR7. On a molar basis, soluble 6Ckine and 6Ckine/mucin-like stalk chimera are essentially equipotent in their ability to chemoattract cultured human lymphocytes.

  1. Haskell, C.A. et al. (2000) J. Biol. Chem. 275:34183.

Customers Who Viewed This Item Also Viewed...

CCL19/MIP-3 beta
[Unconjugated]
361-MI
CCL19/MIP-3 beta [Unconjugated]
(41 Publications)
Species: Hu
Applications: BA
CCR7 Antibody
(150503) [Unconjugate
MAB197
Immunocytochemistry CCR7 Antibody (150503) [Unconjugated]
Immunohistochemistry CCR7 Antibody (150503) [Unconjugated]
Neutralization CCR7 Antibody (150503) [Unconjugated]
(1 Review)
(103 Publications)
Species: Hu
Applications: CyTOF-reported, Dual ISH-IHC, Flow, ICC, IHC, Neut
Gastrin Antibody
NBP3-12223
Immunohistochemistry-Paraffin Gastrin Antibody
Unknown Gastrin Antibody
Species: Hu, Pm, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Doublecortin
Antibody
NBP1-72042
Western Blot Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
(5 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
CXCL13/BLC/BCA-1
Antibody [Unconjugate ...
AF470
Immunohistochemistry CXCL13/BLC/BCA-1 Antibody [Unconjugated]
Neutralization CXCL13/BLC/BCA-1 Antibody [Unconjugated]
(2 Reviews)
(60 Publications)
Species: Mu
Applications: IHC, Neut, WB
CXCL12/SDF-1 alpha
[Unconjugated]
350-NS
SDS-PAGE CXCL12/SDF-1 alpha [Unconjugated]
Bioactivity CXCL12/SDF-1 alpha [Unconjugated]
(189 Publications)
Species: Fe, Hu, RM
Applications: BA, BA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
CCL20/MIP-3 alpha
[HRP]
DM3A00
N/A CCL20/MIP-3 alpha [HRP]
N/A CCL20/MIP-3 alpha [HRP]
N/A CCL20/MIP-3 alpha [HRP]
(53 Publications)
Species: Hu
Applications: ELISA
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(28 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(256 Publications)
Species: Hu
Applications: ELISA
CXCL10/IP-10/CRG-2
[HRP]
DIP100
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
(155 Publications)
Species: Hu
Applications: ELISA
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
CXCR4 Antibody
(44716) [Unconjugated
MAB172
Immunohistochemistry CXCR4 Antibody (44716) [Unconjugated]
Neutralization CXCR4 Antibody (44716) [Unconjugated]
Immunohistochemistry CXCR4 Antibody (44716) [Unconjugated]
(2 Reviews)
(87 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
Somatostatin
Antibody (7G5) - BSA
NBP2-37447
Immunofluorescence Somatostatin Antibody (7G5) - BSA Free
Immunohistochemistry-Paraffin Somatostatin Antibody (7G5) - BSA Free
ELISA Somatostatin Antibody (7G5) - BSA Free
(1 Review)
(3 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(454 Publications)
Species: Hu
Applications: BA
Recombinant Human
CCL21/6Ckine (Mucin S ...
966-6C/CF
Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera), CF
Species: Hu
Applications: Bioactivity

Publications for CCL21/6Ckine (966-6C/CF) (0)

There are no publications for CCL21/6Ckine (966-6C/CF).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CCL21/6Ckine (966-6C/CF) (0)

There are no reviews for CCL21/6Ckine (966-6C/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CCL21/6Ckine (966-6C/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CCL21/6Ckine Products

Blogs on CCL21/6Ckine.

Check out the latest blog posts on CCL21/6Ckine.
Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

CCL21/6Ckine was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
CCL21/6Ckine Antibody [Unconjugated]
AF457
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera), CF and receive a gift card or discount.


Bioinformatics