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Recombinant Human CCL13/MCP-4 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price
Carrier Free
327-P4-025/CF
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Recombinant Human CCL13/MCP-4 Protein Summary

Details of Functionality
Measured by its ability to chemoattract 2-day cultured human monocytes. Matsushima, K. et al. (1989) J. Exp. Med. 169:1485. The ED50 for this effect is 0.02-0.06 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED50 for this effect is 0.5-2 µg/mL.
Source
E. coli-derived human CCL13/MCP-4 protein
Gln24-Thr98
Accession #
N-terminal Sequence
Gln24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL13
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
8.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
327-P4 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile deionized water.  

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL13/MCP-4 Protein

  • C-C motif chemokine 13
  • CCL13
  • chemokine (C-C motif) ligand 13
  • CKb10
  • MCP4
  • MCP-4
  • MCP-4Monocyte chemoattractant protein 4
  • MGC17134
  • NCC-1Monocyte chemotactic protein 4
  • NCC1Small-inducible cytokine A13
  • new CC chemokine 1
  • SCYA13CK-beta-10
  • SCYL1
  • small inducible cytokine subfamily A (Cys-Cys), member 13

Background

Human CCL13 is a CC chemokine recently cloned from a human fetal and a human heart cDNA library. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65 - 66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR-2 and CCR-3, both of which have been shown to bind CCL13.

  1. Garcia-Zepeda, E.A. et al. (1996) J. Immunol. 157:5613.
  2. Uguccioni, M. et al. (1996) J. Exp. Med. 183:2379.
  3. Godisha, R. et al. (1997) J. Leukoc, Biol. 61:353.
  4. Stellato, C. et al. (1997) J. Clin. Invest. 99:926.
  5. Heath, H. et al. (1997) J. Clin. Invest. 99:178.
  6. Charo, I.F. et al. (1994) Proc. Natl. Acad. Sci. USA 91:2752.
  7. Daugherty, B.L. et al. (1996) J. Exp. Med. 183:2349.
  8. Combadiere, C. et al. (1995) J. Biol. Chem. 270:16491.

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Recombinant Human
CCL13/MCP-4 Protein
327-P4
Recombinant Human CCL13/MCP-4 Protein
Species: Hu
Applications: Bioactivity

Publications for CCL13/MCP-4 (327-P4)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, ELISA (Standard).


Filter By Application
Bioassay
(4)
ELISA (Standard)
(1)
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Human
(4)
All Species
Showing Publications 1 - 5 of 5.
Publications using 327-P4 Applications Species
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006-06-16;120(2):220-31. 2006-06-16 [PMID: 16782408] (ELISA (Standard)) ELISA (Standard)
Yamamoto H, Nagata M, Sakamoto Y CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1. Ann. Allergy Asthma Immunol., 2005-02-01;94(2):292-300. 2005-02-01 [PMID: 15765748] (Bioassay, Human) Bioassay Human
Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (Bioassay, Human) Bioassay Human
Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding. Eur. J. Pharmacol., 2002-12-05;456(1):1-10. 2002-12-05 [PMID: 12450563] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol CCL13
Uniprot