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Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera, CF

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When Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera (Catalog # 9660-CD) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) binds with an ED50 of 5-25 ng/mL.
When Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera (Catalog # 9660-CD) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) binds with an ED50 of 5-25 ...read more

Product Details

Summary
Reactivity Pm-CmSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera is coated at 1 μg/mL, 100 μL/well, it binds to Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) with an ED50 of 5-25 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD40/TNFRSF5 protein
Cynomolgus Monkey CD40/TNFRSF5
(Glu21-Arg193)
Accession # XP_005569274.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Glu21
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
57-64 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera, CF

  • B cell surface antigen CD40
  • B-cell surface antigen CD40
  • Bp50B cell-associated molecule
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • CD40 type II isoform
  • CD40
  • CD40L receptor
  • CDw40
  • MGC9013
  • nerve growth factor receptor-related B-lymphocyte activation molecule
  • p50
  • TNFRSF5
  • TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
  • tumor necrosis factor receptor superfamily member 5
  • tumor necrosis factor receptor superfamily, member 5

Background

CD40, also known as TNFRSF5, is a type I transmembrane glycoprotein member of the TNF receptor superfamily (1). Mature Cynomolgus Monkey CD40 consists of an extracellular domain, a transmembrane domain, and a cytoplasmic domain (2). The extracellular domain (21-193 aa) of Cynomolgus Monkey CD40 shares 96%, 58% and 56% amino acid (aa) sequence identity with human, mouse and rat CD40, respectively. CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (4, 5). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40L expressing cells (6). CD40 ligation by CD40L promotes B cell activation and T cell‑dependent humoral responses (7, 8). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (9, 10). Dysregulation of CD40/CD40L expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (11, 12). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (13, 14).
  1. Banchereau, J. et al. (1994) Annu. Rev. Immunol. 12:881.
  2. Stamenkovic, I. et al. (1989) EMBO J. 8:1403.
  3. Eshel, D. et al. (2008) Mol. Immunol. 46:250.
  4. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
  5. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  6. Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  7. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
  8. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
  9. Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
  10. Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
  11. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
  12. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
  13. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
  14. Hassan, G.S. et al. (2012) Immunobiology 217:521.

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FAQs for CD40/TNFRSF5 (9660-CD). (Showing 1 - 2 of 2 FAQ).

  1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
    • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
  2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
    • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

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