![Western Blot: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Rad51C detected in HEK293 lysate using NB 100-177. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Rad51C-Antibody-2H11-6-Azide-and-BSA-Free-Western-Blot-NBP2-80923-img0002.jpg "Western Blot: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Rad51C detected in HEK293 lysate using NB 100-177. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Pm, YeSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | 2H11/6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | His-tagged human Rad51C, over-expressed in E. coli. [UniProt# O43502] |
| Localization | Nuclear |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RAD51C |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In WB, a band can be seen at approx. 40 kDa. Preliminary feedback has been negative for Immunofluorescence on 4% PFA-fixed human cell lines (H1299 and MCF7). In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready. |
| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Rad51C Antibody (NBP2-80923)Find related products by research area.
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| Gene Symbol | RAD51C |
![Flow Cytometry: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Intracellular flow cytometric staining of 1 x 10^6 HeLa cells using Rad51C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Rad51C-Antibody-2H11-6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80923-img0003.jpg "Flow Cytometry: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Intracellular flow cytometric staining of 1 x 10^6 HeLa cells using Rad51C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.")
![Western Blot: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Analysis of RAD51c in 1) Hela WCE 2) HepG2 WCE 3) Cos-7 WCE 4) Hek293 WCE. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Rad51C-Antibody-2H11-6-Azide-and-BSA-Free-Western-Blot-NBP2-80923-img0001.jpg "Western Blot: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Analysis of RAD51c in 1) Hela WCE 2) HepG2 WCE 3) Cos-7 WCE 4) Hek293 WCE. Image from the standard format of this antibody.")
![Simple Western: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Lane view shows a specific band for Rad51C in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Rad51C-Antibody-2H11-6-Azide-and-BSA-Free-Simple-Western-NBP2-80923-img0004.jpg "Simple Western: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Lane view shows a specific band for Rad51C in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.")
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