![Western Blot: Rab27a Antibody (4C1) [H00005873-M01] - RAB27A monoclonal antibody (M01), clone 4C1. Analysis of RAB27A expression in HL-60.](http://images.novusbio.com/fullsize/Rab27a-Antibody-4C1-Western-Blot-H00005873-M01-img0004.jpg "Western Blot: Rab27a Antibody (4C1) [H00005873-M01] - RAB27A monoclonal antibody (M01), clone 4C1. Analysis of RAB27A expression in HL-60.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | 4C1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | RAB27A (NP_004571, 122 a.a. ~ 221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYFETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHASTDQLSEEKEKGACGC |
| Specificity | RAB27A - RAB27A, member RAS oncogene family (4C1) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RAB27A |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00005873-M01 | Applications | Species |
|---|---|---|
| Zhou D, Wei Z, Kuang Z et al. A novel P53/POMC/Galphas/SASH1 autoregulatory feedback loop activates mutated SASH1 to cause pathologic hyperpigmentation. J Cell Mol Med 2016-11-25 [PMID: 27885802] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Rab27a Antibody (H00005873-M01)Find related products by research area.
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![Sandwich ELISA: Rab27a Antibody (4C1) [H00005873-M01] - Detection limit for recombinant GST tagged RAB27A is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Rab27a-Antibody-4C1-Sandwich-ELISA-H00005873-M01-img0003.jpg "Sandwich ELISA: Rab27a Antibody (4C1) [H00005873-M01] - Detection limit for recombinant GST tagged RAB27A is approximately 0.03ng/ml as a capture antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")