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SYTL4 Antibody

Images

 
Genetic Strategies: Western Blot: SYTL4 Antibody [NBP1-86174] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: ...read more
Immunocytochemistry/ Immunofluorescence: SYTL4 Antibody [NBP1-86174] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & microtubule organizing center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SYTL4 Antibody [NBP1-86174] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: SYTL4 Antibody [NBP1-86174] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: SYTL4 Antibody [NBP1-86174] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: SYTL4 Antibody [NBP1-86174] - Staining of human prostate shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: SYTL4 Antibody [NBP1-86174] - Staining of human endometrium shows weak cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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SYTL4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RLKNELLEIKRKGAKRGSQHYSDRTCARCQESLGRLSPKTNTCRGCNHLVCRDCRIQESNGTWRCKVCAKEIELKKATGDWFYDQKVNRFAYRTGSEIIRMSLRHKPAVSKRETVGQSLLHQTQMGDIWPGRKIIQERQKEPSVLFEVP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYTL4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SYTL4 Protein (NBP1-86174PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SYTL4 Antibody

  • DKFZp451P0116
  • exophilin-2
  • FLJ40960
  • Granuphilin
  • granuphilin-a
  • SLP4
  • synaptotagmin-like 4
  • synaptotagmin-like protein 4

Background

Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary.Interacts with vesicles containing negatively charged phospholipids in a Ca(2+)-independent manner

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SYTL4