Orthogonal Strategies: Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Analysis in human cerebral cortex and liver tissues. Corresponding Myosin 5a RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Myosin 5a Antibody [NBP1-92156] - Staining of human cell line A-431 shows localization to microtubule organizing center & focal adhesion sites. Antibody staining shown in green.
Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human kIdney shows moderate memberanous positivity in cells in tubules.
Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human cerebral cortex shows strong membranous positivity in neuropil.
Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human hippocampus shows strong cytoplasmic positivity in pyramidal cells.
Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human kidney shows moderate membranous positivity in cells in tubules.
This antibody was developed against Recombinant Protein corresponding to amino acids: QNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPG
Predicted Species
Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYO5A
Purity
Immunogen affinity purified
Innovator's Reward
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Mouse reactivity reported in scientific literature (PMID:32815283)
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for Myosin 5a Antibody
Dilute myosin heavy chain, non-muscle
GS1myosin, heavy polypeptide kinase
MYH12myosin-12
MYO5
Myosin heavy chain 12
myosin V
myosin VA (heavy chain 12, myoxin)
myosin VA (heavy polypeptide 12, myoxin)
Myosin-12
myosin-Va
myoxin
MYR12
Background
The MYO5 gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The p
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Barthelemy-Requin M, Massey-Harroche D CRB2 controls primary ciliogenesis in association with PAR6 alpha, HOOK2 and RAB8A bioRxiv 2019-11-04 (KD, ICC/IF, Human)
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