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Myosin 5a Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Analysis in human cerebral cortex and liver tissues. Corresponding Myosin 5a RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Myosin 5a Antibody [NBP1-92156] - Staining of human cell line A-431 shows localization to microtubule organizing center & focal adhesion sites. Antibody staining shown in green.
Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human kIdney shows moderate memberanous positivity in cells in tubules.
Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human cerebral cortex shows strong membranous positivity in neuropil.
Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human hippocampus shows strong cytoplasmic positivity in pyramidal cells.
Immunohistochemistry-Paraffin: Myosin 5a Antibody [NBP1-92156] - Staining of human kidney shows moderate membranous positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Myosin 5a Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPG
Predicted Species
Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYO5A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Knockdown Validated Validation (Barthelemy-Requin et al).
Application Notes
IHC-P Retrieval method: HIER pH6, ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
Myosin 5a Protein (NBP1-92156PEP)
Publications
Read Publications using
NBP1-92156 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32815283)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Myosin 5a Antibody

  • Dilute myosin heavy chain, non-muscle
  • GS1myosin, heavy polypeptide kinase
  • MYH12myosin-12
  • MYO5
  • Myosin heavy chain 12
  • myosin V
  • myosin VA (heavy chain 12, myoxin)
  • myosin VA (heavy polypeptide 12, myoxin)
  • Myosin-12
  • myosin-Va
  • myoxin
  • MYR12

Background

The MYO5 gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The p

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin 5a Antibody (NBP1-92156)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IF/IHC, In Vivo, KD.


Filter By Application
ICC/IF
(2)
IF/IHC
(1)
In Vivo
(1)
KD
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP1-92156 Applications Species
Chien-Hui Lo, Zhiquan Liu, Siyu Chen, Frank Lin, Andrew R. Berneshawi, Charles Q. Yu, Euna B. Koo, Tia J. Kowal, Ke Ning, Yang Hu, Won-Jing Wang, Y. Joyce Liao, Yang Sun Primary cilia formation requires the Leigh syndrome–associated mitochondrial protein NDUFAF2 The Journal of Clinical Investigation 2024-07-01 [PMID: 38949024]
Jewett CE, McCurdy BL, O'Toole ET et al. Trisomy 21 induces pericentrosomal crowding delaying primary ciliogenesis and mouse cerebellar development eLife 2023-01-19 [PMID: 36656118] (In Vivo) In Vivo
Rivera-Molina FE, Xi Z, Reales E Et al. Exocyst complex mediates recycling of internal cilia Current biology : CB 2021-10-19 [PMID: 34678163]
Cuenca A, Insinna C, Zhao H et al. The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis J. Biol. Chem. 2019-08-29 [PMID: 31467083] (Human) Human
Reynier M, Allart S, Goudouneche D et al. The Actin-Based Motor Myosin Vb is Crucial to Maintain the Epidermal Barrier Integrity. J. Invest. Dermatol. 2019-01-17 [PMID: 30660668] (Human) Human
Barthelemy-Requin M, Massey-Harroche D CRB2 controls primary ciliogenesis in association with PAR6 alpha, HOOK2 and RAB8A bioRxiv 2019-11-04 (KD, ICC/IF, Human) KD, ICC/IF Human
Stuck MW, Chong WM, Liao JC, Pazour GJ Rab34 is necessary for early stages of intracellular ciliogenesis Current biology : CB 2021-05-07 [PMID: 33989524]
Fan JR, You LR, Wang WJ et al. Lamin A-mediated nuclear lamina integrity is required for proper ciliogenesis EMBO Rep. 2020-08-19 [PMID: 32815283] (IF/IHC, Mouse) IF/IHC Mouse
Lo C H, Lin I H et al. Phosphorylation of CEP83 by TTBK2 is necessary for cilia initiation. J Cell Biol 2019-07-10 [PMID: 31455668] (ICC/IF, Human) ICC/IF Human

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYO5A