![Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - RBL-2H3 from Wt or shRNA Munc13-4 lentiviral stable expression whole cell lysate. 50 ug total protein. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Western-Blot-NB100-41385-img0008.jpg "Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - RBL-2H3 from Wt or shRNA Munc13-4 lentiviral stable expression whole cell lysate. 50 ug total protein. WB image submitted by a verified customer review.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-ETQKHKKDLHPLFD corresponding to internal region according to NP_954712.1. |
| Predicted Species | Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | UNC13D |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Approx. 105 kDa band observed in human T-lymphocyte and HeLa lysates (calculated MW of 123 kDa band according to NP_954712.1). IHC-P: Human thymus shows cytoplasm staining in select areas. Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out, provided by Micheal Tuvim, University of Texas MD Anderson Cancer Center, Houston, USA. Use in ICC/IF reported in scientific literature (PMID 28100639). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Scott Messenger |
WB | Rat | 04/14/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | UNC13D |
| Entrez | |
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![Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of paraffin embedded Mouse Lung with antibody at 0.5 ug/mL (wt in A and KO in B).](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Immunohistochemistry-Paraffin-NB100-41385-img0006.jpg "Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of paraffin embedded Mouse Lung with antibody at 0.5 ug/mL (wt in A and KO in B).")
![Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of Human T-lymphocyte lysate with antibody at 2 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Western-Blot-NB100-41385-img0007.jpg "Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of Human T-lymphocyte lysate with antibody at 2 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of paraffin embedded Human Thymus. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Immunohistochemistry-Paraffin-NB100-41385-img0005.jpg "Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of paraffin embedded Human Thymus. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA were left untreated (Untr) or were treated with TGFbeta-1 for 24 h, and SDS-PAGE Western blotting for indicated proteins was conducted. Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Knockdown-Validated-NB100-41385-img0009.jpg "Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA were left untreated (Untr) or were treated with TGFbeta-1 for 24 h, and SDS-PAGE Western blotting for indicated proteins was conducted. Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY license.")
![Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Knockdown-Validated-NB100-41385-img0010.jpg "Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY license.")
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