Novus Biologicals products are now on bio-techne.com

Pyruvate Dehydrogenase E2 Antibody (SR2133)

Images

 
Western Blot: Pyruvate Dehydrogenase E2 Antibody (SR2133) [NBP3-22153] - Western blot analysis using NBP3-22153 in Jurkat cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IP
Clone
SR2133
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Pyruvate Dehydrogenase E2 Antibody (SR2133) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human Pyruvate Dehydrogenase E2 (Uniprot #: P10515)
Specificity
Detects endogenous levels of total Pyruvate Dehydrogenase E2
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
DLAT
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Pyruvate Dehydrogenase E2 Antibody (SR2133)

  • 70 kDa mitochondrial autoantigen of primary biliary cirrhosis
  • dihydrolipoamide S-acetyltransferase
  • DLTA
  • E2 component of pyruvate dehydrogenase complex
  • EC 2.3.1
  • M2 antigen complex 70 kDa subunit
  • PBC
  • PDC-E2EC 2.3.1.12
  • PDCE2mitochondrial

Background

The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-88347
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-94660
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
202-IL
Species: Hu
Applications: BA
NBP2-93742
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
7268-CT
Species: Hu
Applications: BA
NBP1-31715
Species: Hu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-20383
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
DY417
Species: Mu
Applications: ELISA
M6000B
Species: Mu
Applications: ELISA
NB110-3638
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
NBP2-92749
Species: Hu, Mu
Applications: WB
DPI00
Species: Hu
Applications: ELISA
DCC270
Species: Hu
Applications: ELISA
NBP1-89133
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB

Publications for Pyruvate Dehydrogenase E2 Antibody (NBP3-22153) (0)

There are no publications for Pyruvate Dehydrogenase E2 Antibody (NBP3-22153).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Pyruvate Dehydrogenase E2 Antibody (NBP3-22153) (0)

There are no reviews for Pyruvate Dehydrogenase E2 Antibody (NBP3-22153). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pyruvate Dehydrogenase E2 Antibody (NBP3-22153) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Pyruvate Dehydrogenase E2 Products

Research Areas for Pyruvate Dehydrogenase E2 Antibody (NBP3-22153)

Find related products by research area.

Blogs on Pyruvate Dehydrogenase E2

There are no specific blogs for Pyruvate Dehydrogenase E2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Pyruvate Dehydrogenase E2 Antibody (SR2133) and receive a gift card or discount.

Bioinformatics

Gene Symbol DLAT