Pyruvate Dehydrogenase E2 Antibodies
We offer Pyruvate Dehydrogenase E2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each Pyruvate Dehydrogenase E2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Pyruvate Dehydrogenase E2 Antibodies can be used in a variety of model species: Human, Rat. Use the list below to choose the Pyruvate Dehydrogenase E2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Pyruvate Dehydrogenase E2 polyclonal antibodies.
Alternate Names for Pyruvate Dehydrogenase E2 Antibodies
anti-Pyruvate Dehydrogenase E2 antibody, anti-DLAT antibody, anti-70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody, anti-dihydrolipoamide S-acetyltransferase antibody, anti-DLTA antibody, anti-E2 component of pyruvate dehydrogenase complex antibody, anti-EC 2.3.1 antibody, anti-M2 antigen complex 70 kDa subunit antibody, anti-PBC antibody, anti-PDC-E2EC 2.3.1.12 antibody, anti-PDCE2mitochondrial antibody
12 Results for "pyruvate-dehydrogenase-e2" in Primary Antibodies
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Analysis using Anti-DLAT antibody NBP2-34065 (A) shows similar pattern to independent antibody NBP2-56744 (B).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP2-34065-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Analysis using Anti-DLAT antibody NBP2-34065 (A) shows similar pattern to independent antibody NBP2-56744 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human cell line U-2 OS shows positivity in mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34065-img0005.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human liver shows low expression as expected](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunohistochemistry-Paraffin-NBP2-34065-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Analysis in human heart muscle and liver tissues using Anti-DLAT antibody. Corresponding DLAT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunohistochemistry-Paraffin-NBP2-34065-img0006.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-34065] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunohistochemistry-Paraffin-NBP2-34065-img0007.jpg)
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP2-20026-img0001.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - DLAT antibody detects DLAT protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DLAT protein stained by DLAT antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20026-img0003.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - Immunohistochemical analysis of paraffin-embedded Ovarian carcinoma, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunohistochemistry-Paraffin-NBP2-20026-img0002.jpg)
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Reccomended Titration: 5.0 ug/ml Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP1-54795-img0007.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Reccomended Titration: 5.0 ug/ml Positive Control: Jurkat cell lysate")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunohistochemistry-Paraffin-NBP1-54795-img0005.jpg)
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Sample Type : Huh7 lysate (50ug) Primary Antibody Dilution : 1:200 Image Submitted By : Partha Kasturi University of Kansas Medical Center](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP1-54795-img0002.jpg)
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Sample Tissue: Human Jurkat Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP1-54795-img0006.jpg)
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP2-99340-img0005.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 72 kDa")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Immunochemical staining of human Pyruvate Dehydrogenase E2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunohistochemistry-Paraffin-NBP2-99340-img0003.jpg)
![Immunoprecipitation: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Pyruvate Dehydrogenase E2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 2 ul anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunoprecipitation-NBP2-99340-img0004.jpg)
![Western Blot: Pyruvate Dehydrogenase E2 Antibody (SR2133) [NBP3-22153] - Western blot analysis using NBP3-22153 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-22153_rabbit-pyruvate-dehydrogenase-e2-mab-sr2133-67202310622.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody (SR2133) [NBP3-22153] - Western blot analysis using NBP3-22153 in Jurkat cell lysate.")
![Western Blot: Pyruvate Dehydrogenase E2 Antibody (JE34-38) [NBP3-32880] - Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (NBP3-32880) at 1/500 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: K562 cell lysate Lane 3: Daudi cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32880) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32880_rabbit-pyruvate-dehydrogenase-e2-mab-je34-38-288202412442946.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody (JE34-38) [NBP3-32880] - Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (NBP3-32880) at 1/500 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: K562 cell lysate Lane 3: Daudi cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32880) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Dehydrogenase E2 Antibody (JE34-38) [NBP3-32880] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (NBP3-32880) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32880) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32880_rabbit-pyruvate-dehydrogenase-e2-mab-je34-38-29820241420919.jpg)
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-56744] - Analysis using Anti-DLAT antibody NBP2-56744 (A) shows similar pattern to independent antibody NBP2-34065 (B).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP2-56744-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-56744] - Analysis using Anti-DLAT antibody NBP2-56744 (A) shows similar pattern to independent antibody NBP2-34065 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-56744] - Staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56744-img0001.jpg)
![Western Blot: Pyruvate Dehydrogenase E2 Antibody (2Z7R10) [NBP3-16707] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase E2 Rabbit mAb (NBP3-16707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-2Z7R10-Western-Blot-NBP3-16707-img0001.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody (2Z7R10) [NBP3-16707] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase E2 Rabbit mAb (NBP3-16707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54756] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP1-54756-img0003.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54756] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.")