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DLST Antibody

Images

 
Western Blot: DLST Antibody [NBP1-31715] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLST antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: DLST Antibody [NBP1-31715] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: DLST Antibody [NBP1-31715] - RT2 xenograft, using DLST antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: DLST Antibody [NBP1-31715] - Cal27 xenograft, using DLST antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry: DLST Antibody [NBP1-31715] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using DLST antibody [N3C3] (NBP1-31715) at 1:300 dilution.
Western Blot: DLST Antibody [NBP1-31715] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP1-31715 diluted at 1:1000

Product Details

Summary
Reactivity Hu, Rt, Ze, XpSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DLST Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DLST. The exact sequence is proprietary.
Localization
Mitochondrion
Predicted Species
Xenopus (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLST
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (87%), Xenopus tropicalis (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for DLST Antibody

  • 2-oxoglutarate dehydrogenase complex component E2
  • dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutaratecomplex)
  • Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenasecomplex
  • dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutaratedehydrogenase complex, mitochondrial
  • DLTS
  • E2K
  • EC 2.3.1
  • EC 2.3.1.61
  • OGDC-E2

Background

DLST - dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DLST