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Pygopus-2 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF3616
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF3616
Carrier Free
AF3616
Carrier Free
AF3616-SP
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Pygopus-2 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human Pygopus-2
Met160-Glu336
Accession # Q9BRQ0
Specificity
Detects human Pygopus-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human Pygopus-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PYGO2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Readout System
Publications
Read Publication using
BAF3616 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pygopus-2 Antibody [Biotin]

  • 1190004M21Rik
  • FLJ33226
  • PYGO2
  • pygopus 2
  • pygopus homolog 2 (Drosophila)
  • pygopus homolog 2
  • Pygopus2
  • Pygopus-2

Background

Pygopus-2 is a 41 kDa nuclear protein that functions as a transcriptional co-activator in the Wnt signaling pathway. Human Pygopus-2 is synthesized as a 406 amino acid (aa) residue protein that has the conserved N-terminal homology domain (NHD) and the C-terminal PHD (Plant homeodomain) zinc finger. The PHD zinc finger binds Legless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNA binding proteins. Formulation of the quaternary complex is required for the activation of Wnt-responsive genes. Over the region used for immunization, human Pygopus-2 shares 95% amino acid sequence identity with the mouse protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pygopus-2 Antibody (BAF3616)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol PYGO2
Uniprot