![Western Blot: PUM2 Antibody (JE37-76) [NBP3-32879] - Western blot analysis of PUM2 on different lysates with Rabbit anti-PUM2 antibody (NBP3-32879) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 114 kDa Observed band size: 140 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32879) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32879_rabbit-pum2-mab-je37-76-288202412461618.jpg "Western Blot: PUM2 Antibody (JE37-76) [NBP3-32879] - Western blot analysis of PUM2 on different lysates with Rabbit anti-PUM2 antibody (NBP3-32879) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 114 kDa Observed band size: 140 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32879) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow |
| Clone | JE37-76 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within Human PUM2 aa 1-100. (Uniprot: Q8TB72) |
| Localization | Cytoplasm, Cytoplasmic granule, perinuclear region. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PUM2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 114 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PUM2 Antibody (NBP3-32879)Find related products by research area.
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| Gene Symbol | PUM2 |
![Flow Cytometry: PUM2 Antibody (JE37-76) [NBP3-32879] - Flow cytometric analysis of U-2 OS cells labeling PUM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32879, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32879_rabbit-pum2-mab-je37-76-392024884690.jpg "Flow Cytometry: PUM2 Antibody (JE37-76) [NBP3-32879] - Flow cytometric analysis of U-2 OS cells labeling PUM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32879, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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