PTP1B/PTPN1 [p Ser50] Antibody Summary
Description |
Stable for at least 1 year at -20°C. |
Immunogen |
PTP1B/PTPN1 (Ser-50) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-50 in human PTP1B/PTPN1. This sequence has significant homology to the conserved site in rat and mouse PTP1B/PTPN1, and has homology to conserved sites in TC-PTP and MPTP, but not other protein tyrosine phosphatase family members. [UniProt# P18031] |
Modification |
p Ser50 |
Specificity |
The antibody detects a 50 kDa* doublet corresponding to PTP1B/PTPN1 in western blots of calyculin A treated human A431 and Jurkat cells. This reactivity is observed as a weak singlet in control cells and is not observed after lambda phosphatase treatment. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
PTPN1 |
Purity |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:100
- Western Blot 1:1000
|
Theoretical MW |
50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20C. |
Buffer |
PBS, 1 mg/ml BSA and 50% glycerol |
Preservative |
0.05% Sodium Azide |
Purity |
Antigen Affinity-purified |
Alternate Names for PTP1B/PTPN1 [p Ser50] Antibody
Background
The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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