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PTP1B/PTPN1 Antibody [Unconjugated]

 (14 Publications)
 
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Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.3 µg/mL Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and recombinant mouse PTP1B 5 ng/lane) were included. A specific band for PTP1B was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.3 µg/mL Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by ...read more
Simple Western lane view shows lysates of Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 56 kDa (as indicated) using 5 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3954-SP
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PTP1B/PTPN1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse PTP1B
Met1-Phe431
Accession # P35821
Specificity
Detects mouse and rat PTP1B in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human PTP1B is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PTPN1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 5 ug/mL
  • Western Blot 0.3 ug/mL
Publications
Read Publications using
AF3954 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTP1B/PTPN1 Antibody [Unconjugated]

  • EC 3.1.3.48
  • protein tyrosine phosphatase 1B
  • protein tyrosine phosphatase, non-receptor type 1
  • Protein-tyrosine phosphatase 1B
  • PTP1B
  • PTP-1B
  • PTP1Bprotein tyrosine phosphatase, placental
  • PTPN1
  • tyrosine-protein phosphatase non-receptor type 1

Background

Protein tyrosine phosphatase 1B (PTP1B), also known as protein tyrosine phosphatase, non-receptor type 1 (PTPN1) and placental protein tyrosine phosphatase, dephosphorylates tyrosine residues in proteins. PTP1B is a cytosolic enzyme anchored in the endoplasmic reticulum that plays a critical role in regulating growth factor responsiveness. PTP1B knockout mice show increased resistance to high fat diet-induced obesity and have exaggerated responses to insulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PTP1B/PTPN1 Antibody [Unconjugated]
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Publications for PTP1B/PTPN1 Antibody (AF3954)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, IHC-Fr, Western Blot.


Filter By Application
IHC
(1)
IHC-Fr
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Rat
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF3954 Applications Species
Yong Liu, Jing Zhang, Zefan Du, Junbin Huang, Yucai Cheng, Wenfang Yi et al. Comprehensive analysis of PTPN family expression and prognosis in acute myeloid leukemia Frontiers in Genetics 2023-01-09 [PMID: 36699453]
Yoshihiro Ito, Runan Sun, Hiroshi Yagimuma, Keigo Taki, Akira Mizoguchi, Tomoko Kobayashi et al. Protein Tyrosine Phosphatase 1B Deficiency Improves Glucose Homeostasis in Type 1 Diabetes Treated With Leptin Diabetes 2022-09-01 [PMID: 35748319]
Florian Wiede, Kun-Hui Lu, Xin Du, Mara N. Zeissig, Rachel Xu, Pei Kee Goh et al. PTP1B Is an Intracellular Checkpoint that Limits T-cell and CAR T-cell Antitumor Immunity Cancer Discovery 2022-03-01 [PMID: 34794959]
Beatriz Ferrer, Harshini Suresh, Abel Santamaria, João Batista Rocha, Aaron B. Bowman, Michael Aschner The antioxidant role of STAT3 in methylmercury-induced toxicity in mouse hypothalamic neuronal GT1-7 cell line Free Radical Biology and Medicine 2021-08-01 [PMID: 34010664]
Beatriz Ferrer, Lisa M. Prince, Alexey A. Tinkov, Abel Santamaria, Marcelo Farina, João Batista Rocha et al. Chronic exposure to methylmercury enhances the anorexigenic effects of leptin in C57BL/6J male mice Food and Chemical Toxicology 2021-01-01 [PMID: 33338554]
MF Hsu, S Koike, A Mello, LE Nagy, FG Haj Hepatic protein-tyrosine phosphatase 1B disruption and pharmacological inhibition attenuate ethanol-induced oxidative stress and ameliorate alcoholic liver disease in mice Redox Biol, 2020-07-24;36(0):101658. 2020-07-24 [PMID: 32769011] (IHC, Western Blot, Human, Mouse) IHC, Western Blot Human, Mouse
GT Dodd, CE Xirouchaki, M Eramo, CA Mitchell, ZB Andrews, BA Henry, MA Cowley, T Tiganis Intranasal Targeting of Hypothalamic PTP1B and TCPTP Reinstates Leptin and Insulin Sensitivity and Promotes Weight Loss in Obesity Cell Rep, 2019-09-10;28(11):2905-2922.e5. 2019-09-10 [PMID: 31509751] (Western Blot, Mouse) Western Blot Mouse
Ernane Torres Uchoa, Paula Beatriz Marangon, Rodrigo Rorato, Silvia Graciela Ruginsk, Lucas Kniess Debarba, Jose Antunes-Rodrigues et al. Adrenalectomy impairs insulin-induced hypophagia and related hypothalamic changes Journal of Endocrinology 2019-08-01 [PMID: 31189132]
Marcus Grohmann, Florian Wiede, Garron T. Dodd, Esteban N. Gurzov, Geraldine J. Ooi, Tariq Butt et al. Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCC Cell 2018-11-15 [PMID: 30454647]
Y Xu, P Taylor, J Andrade, B Ueberheide, B Shuch, PM Glazer, RS Bindra, MF Moran, WM Linehan, BG Neel Pathological Oxidation of PTPN12 Underlies ABL1 Phosphorylation in Hereditary Leiomyomatosis and Renal Cell Carcinoma Cancer Res., 2018-10-08;0(0):. 2018-10-08 [PMID: 30297534] (Western Blot, Rat) Western Blot Rat
Show All 14 Publications.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PTPN1
Uniprot