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PPP2CB Antibody - Azide and BSA Free

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Western Blot: PPP2CB Antibody [NBP2-94074] - Analysis of extracts of various cell lines, using PPP2CB at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug ...read more
Immunocytochemistry/ Immunofluorescence: PPP2CB Antibody [NBP2-94074] - Analysis of U-2 OS cells using PPP2CB at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PPP2CB Antibody [NBP2-94074] - Paraffin-embedded rat kidney using PPP2CB .
Immunohistochemistry-Paraffin: PPP2CB Antibody [NBP2-94074] - Paraffin-embedded human colon carcinoma using PPP2CB .
Immunohistochemistry-Paraffin: PPP2CB Antibody [NBP2-94074] - Paraffin-embedded mouse kidney using PPP2CB .

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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PPP2CB Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PPP2CB (NP_001009552.1). MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP2CB
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for PPP2CB Antibody - Azide and BSA Free

  • EC 3.1.3.16
  • PP2Abeta
  • PP2A-beta
  • PP2CB
  • protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
  • protein phosphatase 2, catalytic subunit, beta isoform
  • protein phosphatase 2, catalytic subunit, beta isozyme
  • protein phosphatase 2A catalytic subunit, beta isoform
  • protein phosphatase type 2A catalytic subunit
  • serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform
  • serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

Background

PPP2CB also known as Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, is a 309 amino acid protein that is 36 kDa, localizes in cytoplasm and nucleus, is implicated in the negative control of cell growth and division and modulates the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Studies of this protein are being done on the following diseases and disorders: cerebral cavernous malformations, cavernous malformation, cerebral cavernous malformations 3, Werner syndrome, Chagas disease, hepatitis c, trypanosomiasis, cerebritis, Alzheimer's disease, prostate cancer, hepatitis, prostatitis, immunodeficiency, malaria, neuronitis, and breast cancer. PPP2CB protein has been linked to Neurophysiological process Glutamate regulation of Dopamine D1A receptor signaling, Signal transduction Calcium signaling, Development Beta-adrenergic receptors signaling via cAMP,Cell cycle Regulation of G1/S transition (part 1), Cell cycle Start of DNA replication in early S phase, Cell Cycle Control by BTG Proteins, CDK5 Pathway, mTOR Pathway, AMPK Enzyme Complex Pathway, Cyclins and Cell Cycle Regulation, Akt Pathway, Insulin Pathway, TGF-beta Pathway, Wnt Pathway, Apoptosis and survival BAD phosphorylation, and many other pathways where interacts with HIST1H4B , HIST1H4A, HIST1H4C, HIST1H4D, HIST1H4E, AXIN2, and over 280 other interacting proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PPP2CB