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POU3F3 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: POU3F3 Antibody [NBP2-49508] - Analysis in human kidney and pancreas tissues. Corresponding RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: POU3F3 Antibody [NBP2-49508] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: POU3F3 Antibody [NBP2-49508] - Staining of human kidney shows moderate to strong nuclear positivity in cells in distal tubules.
Immunohistochemistry-Paraffin: POU3F3 Antibody [NBP2-49508] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in granular layer.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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POU3F3 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GGAQSLVHPGLVRGDTPELAEHHHHH
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POU3F3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
POU3F3 Recombinant Protein Antigen (NBP2-49508PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for POU3F3 Antibody

  • brain-1
  • Brain-specific homeobox/POU domain protein 1
  • Brn-1
  • BRN1brn-1
  • Oct-8
  • Octamer-binding protein 8
  • Octamer-binding transcription factor 8
  • OTF-8
  • OTF8oct-8
  • POU class 3 homeobox 3
  • POU domain class 3, transcription factor 3
  • POU domain, class 3, transcription factor 3

Background

POU3F3, also known as POU Class 3 Homeobox 3, is a transcription factor that is primarily expressed in the brain and CNS. POU3F3 interacts with SOX4 and SOX11, and is implicated in the development of neurons. Current research surrounding POU3F3 has shown potential linkages with neuronitis and cerebritis. POU3F3 has also been shown to interact with POU3F4, CAV1, SOX10, and GADD34.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol POU3F3