I need to know about TNF-alpha antibody kits for mice.

We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.

I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.

I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.

I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?

The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.

Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?

I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Novus Biologicals products are now on bio-techne.com

Porcine TNF-alpha Quantikine ELISA Kit

Name: Porcine TNF-alpha Quantikine ELISA Kit
Brand: Novus Biologicals
SKU: PTA00
Price: 635 USD

(85 Publications)

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Summary

Reactivity	PoSpecies Glossary
Applications	ELISA
Conjugate	HRP

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Porcine TNF-alpha Quantikine ELISA Kit Summary

Background	The Quantikine Porcine TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine TNF-alpha in cell culture supernates, serum, and plasma samples. It contains E. coli-expressed recombinant porcine TNF-alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately measure recombinant porcine TNF-alpha. Results obtained... using natural porcine TNF-alpha show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine TNF-alpha. Show More
Specificity	Natural and recombinant porcine TNF-alpha
Source	N/A
Assay Type	Solid Phase Sandwich ELISA
Inter-Assay	See PDF Datasheet for details
Intra-Assay	See PDF Datasheet for details
Spike Recovery	See PDF Datasheet for details
Sample Volume	See PDF Datasheet for details
Gene	TNF

Applications/Dilutions

Dilutions

ELISA

Application Notes

No significant interference observed with available related molecules.

Publications

Read Publications using
PTA00 in the following applications:

ELISA Capture
1 publication

Packaging, Storage & Formulations

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Porcine TNF-alpha Quantikine ELISA Kit

APC1 protein
Cachectin
Cachetin
DIF
TNF
TNF, monocyte-derived
TNFA
TNF-A
TNFalpha
TNF-alpha
TNF-alphacachectin
TNFATNF, macrophage-derived
TNFG1F
TNFSF1A
TNFSF2
TNFSF2TNF superfamily, member 2
tumor necrosis factor (TNF superfamily, member 2)
tumor necrosis factor alpha
Tumor necrosis factor ligand superfamily member 2
tumor necrosis factor
tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

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Porcine TNF-alpha
Quantikine ELISA Kit

PTA00

Species: Po

Applications: ELISA

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TNF-alpha (PTA00)(85)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: ELISA Capture.

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ELISA Capture

(1)

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Porcine

(85)

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Showing Publications 1 - 10 of 85. Show All 85 Publications.

Publications using PTA00	Applications	Species
Zhou, B;Xiao, K;Guo, J;Xu, Q;Xu, Q;Lv, Q;Zhu, H;Zhao, J;Liu, Y; Necroptosis contributes to the intestinal toxicity of deoxynivalenol and is mediated by methyltransferase SETDB1 Journal of hazardous materials 2024-08-05 [PMID: 38823098] (Porcine)		Porcine
Paslawski, R;Kowalczyk, P;Paslawska, U;Wi?niewski, J;Dzi?giel, P;Janiszewski, A;Kiczak, L;Zacharski, M;Gawdzik, B;Kramkowski, K;Szuba, A; Analysis of the Model of Atherosclerosis Formation in Pig Hearts as a Result of Impaired Activity of DNA Repair Enzymes International journal of molecular sciences 2024-02-14 [PMID: 38396961] (Porcine)		Porcine
Silva-Guillen, YV;Arellano, C;Wiegert, J;Boyd, RD;Martínez, GE;van Heugten, E; Supplementation of vitamin E or a botanical extract as antioxidants to improve growth performance and health of growing pigs housed under thermoneutral or heat-stressed conditions Journal of animal science and biotechnology 2024-02-19 [PMID: 38369504] (Porcine)		Porcine
Fardisi, M;Thelen, K;Groenendal, A;Rajput, M;Sebastian, K;Contreras, GA;Moeser, AJ; Early weaning and biological sex shape long-term immune and metabolic responses in pigs Scientific reports 2023-09-23 [PMID: 37741873] (Porcine)		Porcine
Yang, X;Du, Q;Wang, X;Shi, J;Wang, T;Li, P;Zhong, J;Tong, D;Huang, Y; Porcine circovirus type 2 infection inhibits macrophage M1 polarization induced by other pathogens via viral capsid protein and host gC1qR protein Veterinary microbiology 2023-08-31 [PMID: 37672899] (Porcine)		Porcine
Lin, S;Panthi, S;Hsuuw, Y;Chen, S;Huang, M;Sieber, M;Hsuuw, Y; Regenerative Effect of Mesenchymal Stem Cell on Cartilage Damage in a Porcine Model Biomedicines 2023-01-01 [PMID: 37509451] (Porcine)		Porcine
Deng, Z;Jang, KB;Jalukar, S;Du, X;Kim, SW; Efficacy of Feed Additive Containing Bentonite and Enzymatically Hydrolyzed Yeast on Intestinal Health and Growth of Newly Weaned Pigs under Chronic Dietary Challenges of Fumonisin and Aflatoxin Toxins 2023-06-30 [PMID: 37505702] (Porcine)		Porcine
Changsan, N;Sawatdee, S;Suedee, R;Chunhachaichana, C;Srichana, T; Aqueous cannabidiol ?-cyclodextrin complexed polymeric micelle nasal spray to attenuate in vitro and ex vivo SARS-CoV-2-induced cytokine storms International journal of pharmaceutics 2023-05-12 [PMID: 37182795] (Porcine)		Porcine
SY Chang, MH Song, JH Lee, HJ Oh, YJ Kim, JW An, YB Go, DC Song, HA Cho, SY Cho, DJ Kim, MS Kim, HB Kim, JH Cho Phytogenic feed additives alleviate pathogenic Escherichia coli-induced intestinal damage through improving barrier integrity and inhibiting inflammation in weaned pigs Journal of animal science and biotechnology, 2022-09-02;13(1):107. 2022-09-02 [PMID: 36050784] (Porcine)		Porcine
R Rissel, M Gosling, J Kamuf, M Renz, R Ruemmler, A Ziebart, EK Hartmann Levosimendan Ameliorates Cardiopulmonary Function but Not Inflammatory Response in a Dual Model of Experimental ARDS Biomedicines, 2022-04-29;10(5):. 2022-04-29 [PMID: 35625767] (Porcine)		Porcine
G Vilahur, P Sutelman, S Ben-Aicha, G Mendieta, M Radiké, L Schoch, L Casaní, M Borrell-Pa, T Padro, L Badimon Supplementation With Spirulina Reduces Infarct Size and Ameliorates Cardiac Function in a Pig Model of STEMI Frontiers in Pharmacology, 2022-05-03;13(0):891801. 2022-05-03 [PMID: 35592428] (Porcine)		Porcine
DM Holanda, SW Kim Impacts of weaning weights and mycotoxin challenges on jejunal mucosa-associated microbiota, intestinal and systemic health, and growth performance of nursery pigs Journal of animal science and biotechnology, 2022-04-13;13(1):43. 2022-04-13 [PMID: 35413935] (Porcine)		Porcine
A Rodríguez-, L Castillejo, P López-Colo, G Cifuentes-, JA Moreno-Muñ, SM Martín-Orú Assessment of the Effects of the Synbiotic Combination of Bifidobacterium longum subsp. infantis CECT 7210 and Oligofructose-Enriched Inulin Against Digestive Bacterial Infections in a Piglet Model Frontiers in Microbiology, 2022-03-08;13(0):831737. 2022-03-08 [PMID: 35350617] (Porcine)		Porcine
M Kasztura, L Kiczak, U Pas?awska, J Bania, A Janiszewsk, A Tomaszek, M Zacharski, A Noszczyk-N, R Pas?awski, A Tabi?, P Kuropka, P Dzi?giel, P Ponikowski Hemosiderin Accumulation in Liver Decreases Iron Availability in Tachycardia-Induced Porcine Congestive Heart Failure Model International Journal of Molecular Sciences, 2022-01-18;23(3):. 2022-01-18 [PMID: 35162949] (Porcine)		Porcine
HJ Lee, HD Kim, CH Jo, EY Bok, SB Kim, SL Lee, M Jang, SG Bae, SH Yun, SJ Kim, GJ Rho, WJ Lee IFN-gamma Licensing Does Not Enhance the Reduced Immunomodulatory Potential and Migratory Ability of Differentiation-Induced Porcine Bone Marrow-Derived Mesenchymal Stem Cells in an In Vitro Xenogeneic Application BioMed Research International, 2021-09-04;2021(0):4604856. 2021-09-04 [PMID: 34527737] (Porcine)		Porcine
J Kamuf, A Garcia Bar, A Ziebart, R Ruemmler, J Schwab, M Dib, A Daiber, SC Thal, EK Hartmann Influence of rosuvastatin treatment on cerebral inflammation and nitro-oxidative stress in experimental lung injury in pigs BMC anesthesiology, 2021-09-13;21(1):224. 2021-09-13 [PMID: 34517845] (Porcine)		Porcine
DM Holanda, YI Kim, W Parnsen, SW Kim Phytobiotics with Adsorbent to Mitigate Toxicity of Multiple Mycotoxins on Health and Growth of Pigs Toxins, 2021-06-26;13(7):. 2021-06-26 [PMID: 34206784] (Porcine)		Porcine
X Qu, C Neuhoff, MU Cinar, M Pröll, E Tholen, D Tesfaye, M Hölker, K Schellande, MJ Uddin Epigenetic Modulation of TLR4 Expression by Sulforaphane Increases Anti-Inflammatory Capacity in Porcine Monocyte-Derived Dendritic Cells Biology, 2021-05-31;10(6):. 2021-05-31 [PMID: 34072812] (Porcine)		Porcine
A Rodríguez-, L Castillejo, P López-Colo, G Cifuentes-, M Rodríguez-, JA Moreno-Muñ, D Luise, P Trevisi, SM Martín-Orú Effects of the Administration of Bifidobacterium longum subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001 and Their Synbiotic Combination With Galacto-Oligosaccharides Against Enterotoxigenic Escherichia coli F4 in an Early Weaned Piglet Model Frontiers in Microbiology, 2021-04-16;12(0):642549. 2021-04-16 [PMID: 33935999] (Porcine)		Porcine
M Al-Obeidal, D Jarkovská, L Valešová, J Horák, J Jedli?ka, L Nalos, J Chvojka, J Švíglerová, J Kuncová, J Beneš, M Mat?jovi?, M Štengl SOFA Score, Hemodynamics and Body Temperature Allow Early Discrimination between Porcine Peritonitis-Induced Sepsis and Peritonitis-Induced Septic Shock Journal of personalized medicine, 2021-02-28;11(3):. 2021-02-28 [PMID: 33670874] (Porcine)		Porcine
J Bhogal, AL Solevåg, M O'Reilly, TF Lee, C Joynt, LK Hornberger, GM Schmölzer, PY Cheung Hemodynamic effects of high frequency oscillatory ventilation with volume guarantee in a piglet model of respiratory distress syndrome PLoS ONE, 2021-02-16;16(2):e0246996. 2021-02-16 [PMID: 33592023] (Porcine)		Porcine
J Kamuf, A Garcia Bar, A Ziebart, K Frauenknec, K Folkert, J Schwab, R Ruemmler, M Renz, D Cana, SC Thal, EK Hartmann Experimental lung injury induces cerebral cytokine mRNA production in pigs PeerJ, 2020-12-09;8(0):e10471. 2020-12-09 [PMID: 33354426] (Porcine)		Porcine
A Toschi, B Tugnoli, B Rossi, A Piva, E Grilli Thymol modulates the endocannabinoid system and gut chemosensing of weaning pigs BMC Vet. Res., 2020-08-12;16(1):289. 2020-08-12 [PMID: 32787931] (Porcine)		Porcine
DM Holanda, A Yiannikour, SW Kim Investigation of the Efficacy of a Postbiotic Yeast Cell Wall-Based Blend on Newly-Weaned Pigs under a Dietary Challenge of Multiple Mycotoxins with Emphasis on Deoxynivalenol Toxins (Basel), 2020-08-06;12(8):. 2020-08-06 [PMID: 32781569] (Porcine)		Porcine
YT Hsu, CH Wu, CY Chao, YS Wei, YC Chang, YT Chen, SL Lin, SY Tsai, YJ Lee, PS Tsai Hypochlorite-induced porcine model of peritoneal fibrosis through the activation of IL1&beta-CX3CL1-TGF&beta1 signal axis Sci Rep, 2020-07-13;10(1):11496. 2020-07-13 [PMID: 32661265] (Porcine)		Porcine
GH Shim, SY Kim, PY Cheung, TF Lee, M O'Reilly, GM Schmölzer Effects of sustained inflation pressure duringï¿½neonatal cardiopulmonary resuscitation of asphyxiated piglets PLoS ONE, 2020-06-23;15(6):e0228693. 2020-06-23 [PMID: 32574159] (Porcine)		Porcine
DM Holanda, SW Kim Efficacy of Mycotoxin Detoxifiers on Health and Growth of Newly-Weaned Pigs under Chronic Dietary Challenge of Deoxynivalenol Toxins (Basel), 2020-05-09;12(5):. 2020-05-09 [PMID: 32397551] (Porcine)		Porcine
S Spring, H Premathila, U DeSilva, C Shili, S Carter, A Pezeshki Low Protein-High Carbohydrate Diets Alter Energy Balance, Gut Microbiota Composition and Blood Metabolomics Profile in Young Pigs Sci Rep, 2020-02-24;10(1):3318. 2020-02-24 [PMID: 32094453] (Porcine)		Porcine
ME Duarte, FX Zhou, WM Dutra, SW Kim Dietary supplementation of xylanase and protease on growth performance, digesta viscosity, nutrient digestibility, immune and oxidative stress status, and gut health of newly weaned pigs Anim Nutr, 2019-05-11;5(4):351-358. 2019-05-11 [PMID: 31890911] (Porcine)		Porcine
P López-Colo, L Castillejo, A Rodríguez-, M Puyalto, JJ Mallo, SM Martín-Orú Efficacy of medium-chain fatty acid salts distilled from coconut oil against two enteric pathogen challenges in weanling piglets J Anim Sci Biotechnol, 2019-11-09;10(0):89. 2019-11-09 [PMID: 31728192] (Porcine)		Porcine
SE Pischke, S Hestenes, HT Johansen, H Fure, JF Bugge, A Espinoza, H Skulstad, T Edvardsen, E Fosse, TE Mollnes, PS Halvorsen, EW Nielsen Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model PLoS ONE, 2019-06-27;14(6):e0218624. 2019-06-27 [PMID: 31247004] (Porcine)		Porcine
Y Luo, J Xu, C Zhang, C Jiang, Y Ma, H He, Y Wu, B Devriendt, E Cox, H Zhang Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection Vet. Res., 2019-06-20;50(1):48. 2019-06-20 [PMID: 31221216] (Porcine)		Porcine
P López-Colo, K Yu, E Barba-Vida, Y Saco, SM Martín-Orú, L Castillejo, D Solà-Oriol, A Bassols I-FABP, Pig-MAP and TNF-? as biomarkers for monitoring gut-wall integrity in front of Salmonella Typhimurium and ETEC K88 infection in a weaned piglet model Res. Vet. Sci., 2019-05-07;124(0):426-432. 2019-05-07 [PMID: 31082572] (Porcine)		Porcine
Y Liu, R Li, XX Chen, Y Zhi, R Deng, EM Zhou, S Qiao, G Zhang Nonmuscle Myosin Heavy Chain IIA Recognizes Sialic Acids on Sialylated RNA Viruses To Suppress Proinflammatory Responses via the DAP12-Syk Pathway MBio, 2019-05-07;10(3):. 2019-05-07 [PMID: 31064828] (Porcine)		Porcine
R Saleri, V Cavalli, L Ferrari, G Ogno, E Canelli, P Martelli, P Borghetti Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP-PRRSV-1) infection Res. Vet. Sci., 2019-04-11;124(0):263-269. 2019-04-11 [PMID: 31003008] (Porcine)		Porcine
S Boehme, EK Hartmann, T Tripp, SC Thal, M David, D Abraham, JE Baumgardne, K Markstalle, KU Klein PO2 oscillations induce lung injury and inflammation Crit Care, 2019-03-27;23(1):102. 2019-03-27 [PMID: 30917851] (Porcine)		Porcine
L Zhang, X Wang, S Chen, S Wang, Z Tu, G Zhang, H Zhu, X Li, J Xiong, Y Liu Medium-Chain Triglycerides Attenuate Liver Injury in Lipopolysaccharide-Challenged Pigs by Inhibiting Necroptotic and Inflammatory Signaling Pathways Int J Mol Sci, 2018-11-21;19(11):. 2018-11-21 [PMID: 30469452] (Porcine)		Porcine
A Miko?ajczy, D Z?otkowska Neuroimmunological Implications of Subclinical Lipopolysaccharide from Salmonella Enteritidis Int J Mol Sci, 2018-10-22;19(10):. 2018-10-22 [PMID: 30360353] (Porcine)		Porcine
Q Qin, X Xu, X Wang, H Wu, H Zhu, Y Hou, B Dai, X Liu, Y Liu Glutamate alleviates intestinal injury, maintains mTOR and suppresses TLR4 and NOD signaling pathways in weanling pigs challenged with lipopolysaccharide Sci Rep, 2018-10-11;8(1):15124. 2018-10-11 [PMID: 30310102] (Porcine)		Porcine
M Sadeghi, TM Hörer, D Forsman, EM Dogan, K Jansson, C Kindler, P Skoog, KF Nilsson Blood pressure targeting by partial REBOA is possible in severe hemorrhagic shock in pigs and produces less circulatory, metabolic and inflammatory sequelae than total REBOA Injury, 2018-09-29;0(0):. 2018-09-29 [PMID: 30301556] (Porcine)		Porcine
A Bergmann, T Schilling, G Hedenstier, K Ahlgren, A Larsson, M Kretzschma, A Kozian, T Hachenberg Pulmonary Effects of Remote Ischemic Preconditioning in a Porcine Model of Ventilation-induced Lung Injury Respir Physiol Neurobiol, 2018-08-31;0(0):. 2018-08-31 [PMID: 30176289] (Porcine)		Porcine
M Kaiser, M Jacobson, PH Andersen, P Bækbo, JJ Cerón, J Dahl, D Escribano, S Jacobsen Inflammatory markers before and after farrowing in healthy sows and in sows affected with postpartum dysgalactia syndrome BMC Vet. Res., 2018-03-12;14(1):83. 2018-03-12 [PMID: 29530043] (Porcine)		Porcine
X Xu, S Chen, H Wang, Z Tu, S Wang, X Wang, H Zhu, C Wang, J Zhu, Y Liu Medium-chain TAG improve intestinal integrity by suppressing toll-like receptor 4, nucleotide-binding oligomerisation domain proteins and necroptosis signalling in weanling piglets challenged with lipopolysaccharide Br. J. Nutr., 2018-03-06;0(0):1-10. 2018-03-06 [PMID: 29508680] (Porcine)		Porcine
T Duburcq, A Durand, A Tournoys, V Gnemmi, V Gmyr, F Pattou, M Jourdain, F Tamion, E Besnier, S Préau, E Parmentier, D Mathieu, J Poissy, R Favory Sodium lactate improves renal microvascular thrombosis compared to sodium bicarbonate and 0.9% NaCl in a porcine model of endotoxic shock: an experimental randomized open label controlled study Ann Intensive Care, 2018-02-14;8(1):24. 2018-02-14 [PMID: 29445877] (Porcine)		Porcine
V Kloubert, K Blaabjerg, TS Dalgaard, HD Poulsen, L Rink, I Wessels Influence of zinc supplementation on immune parameters in weaned pigs J Trace Elem Med Biol, 2018-01-31;0(0):. 2018-01-31 [PMID: 29402502] (ELISA Capture, Porcine)	ELISA Capture	Porcine
W Gozdzik, S Zielinski, M Zielinska, K Ratajczak, P Skrzypczak, S Rodziewicz, A Kübler, K Löfström, P Dziegiel, M Olbromski, B Adamik, S Ryniak, P Harbut, J Albert, C Frostell Beneficial effects of inhaled nitric oxide with intravenous steroid in an ischemia-reperfusion model involving aortic clamping Int J Immunopathol Pharmacol, 2018-01-01;32(0):3946320177514. 2018-01-01 [PMID: 29376749] (Porcine)		Porcine
H Yan, KM Ajuwon Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway PLoS ONE, 2017-06-27;12(6):e0179586. 2017-06-27 [PMID: 28654658] (Porcine)		Porcine
M Kern, D Günzel, JR Aschenbach, K Tedin, A Bondzio, U Lodemann Altered Cytokine Expression and Barrier Properties after In Vitro Infection of Porcine Epithelial Cells with Enterotoxigenic Escherichia coli and Probiotic Enterococcus faecium Mediators Inflamm., 2017-05-14;2017(0):2748192. 2017-05-14 [PMID: 28607532] (Porcine)		Porcine
D Lukovic, K Zlabinger, A Gugerell, A Spannbauer, N Pavo, L Mandic, DT Weidenauer, S Kastl, C Kaun, A Posa, I Sabdyushev, J Winkler, M Gyöngyösi Inhibition of CD34+ cell migration by matrix metalloproteinase-2 during acute myocardial ischemia, counteracted by ischemic preconditioning F1000Res, 2016-11-22;5(0):2739. 2016-11-22 [PMID: 28299177] (Porcine)		Porcine
Angela J Westover An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome J Diabetes Res, 2016-10-13;2016(0):3486727. 2016-10-13 [PMID: 27819007] (Porcine)		Porcine
Rolf Rossaint Septic porcine blood does not further activate coagulation during in vitro membrane oxygenation Eur J Cardiothorac Surg, 2017-03-01;0(0):. 2017-03-01 [PMID: 27806995] (Porcine)		Porcine
Hartmann E, Ziebart A, Thomas R, Liu T, Schad A, Tews M, Moosmann B, Kamuf J, Duenges B, Thal S, David M Inhalation therapy with the synthetic TIP-like peptide AP318 attenuates pulmonary inflammation in a porcine sepsis model. BMC Pulm Med, 2015-02-07;15(0):7. 2015-02-07 [PMID: 25879802] (Porcine)		Porcine
Qu X, Proll M, Neuhoff C, Zhang R, Cinar M, Hossain M, Tesfaye D, Grosse-Brinkhaus C, Salilew-Wondim D, Tholen E, Looft C, Holker M, Schellander K, Uddin M Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide. PLoS ONE, 2015-03-20;10(3):e0121574. 2015-03-20 [PMID: 25793534] (Porcine)		Porcine
Clayton K, Douglas-Vail M, Nur-ur Rahman A, Medcalf K, Xie I, Chew G, Tandon R, Lanteri M, Norris P, Deeks S, Ndhlovu L, Ostrowski M Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression. J Virol, 2015-01-21;89(7):3723-36. 2015-01-21 [PMID: 25609823] (Porcine)		Porcine
Ziebart A, Hartmann E, Thomas R, Liu T, Duenges B, Schad A, Bodenstein M, Thal S, David M Low tidal volume pressure support versus controlled ventilation in early experimental sepsis in pigs. Respir Res, 2014-09-06;15(0):101. 2014-09-06 [PMID: 25189285] (Porcine)		Porcine
Li XQ, Zhu YH, Zhang HF, Yue Y, Cai ZX, Lu QP, Zhang L, Weng XG, Zhang FJ, Zhou D, Yang JC, Wang JF Risks Associated with High-Dose Lactobacillus rhamnosus in an Escherichia coli Model of Piglet Diarrhoea: Intestinal Microbiota and Immune Imbalances. PLoS ONE, 2012-07-27;7(7):e40666. 2012-07-27 [PMID: 22848393] (Porcine)		Porcine
Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig. Mol. Ther., 2012-03-27;20(6):1204-11. 2012-03-27 [PMID: 22453765] (Porcine)		Porcine
Barratt-Due A, Thorgersen EB, Lindstad JK, Pharo A, Lissina O, Lambris JD, Nunn MA, Mollnes TE Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans. J. Immunol., 2011-09-30;187(9):4913-9. 2011-09-30 [PMID: 21964028] (Porcine)		Porcine
Martinez-Olondris P, Rigol M, Soy D, Guerrero L, Agusti C, Quera MA, Li Bassi G, Esperatti M, Luque N, Liapikou M, Filella X, Marco F, de la Bellacasa JP, Torres A Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs. Crit. Care Med., 2012-01-01;40(1):162-8. 2012-01-01 [PMID: 21926613] (Porcine)		Porcine
Hellerud BC, Nielsen EW, Thorgersen EB, Lindstad JK, Pharo A, Tonnessen TI, Castellheim A, Mollnes TE, Brandtzaeg P Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis. Crit. Care Med., 2010-06-01;38(6):1467-74. 2010-06-01 [PMID: 20400898] (Porcine)		Porcine
McILwain RB, Timpa JG, Kurundkar AR Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine. Lab. Invest., 2009-11-09;90(1):128-39. 2009-11-09 [PMID: 19901912] (Porcine)		Porcine
Thorgersen EB, Hellerud BC, Nielsen EW, Barratt-Due A, Fure H, Lindstad JK, Pharo A, Fosse E, Tonnessen TI, Johansen HT, Castellheim A, Mollnes TE CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs. FASEB J., 2009-10-19;24(3):712-22. 2009-10-19 [PMID: 19841036] (Porcine)		Porcine
Niemann JT, Rosborough JP, Youngquist S, Shah AP, Lewis RJ, Phan QT, Filler SG Cardiac Function and the Proinflammatory Cytokine Response After Recovery From Cardiac Arrest in Swine. J. Interferon Cytokine Res., 2009-11-01;0(0):. 2009-11-01 [PMID: 19642909] (Porcine)		Porcine
Schuleri KH, Feigenbaum GS, Centola M, Weiss ES, Zimmet JM, Turney J, Kellner J, Zviman MM, Hatzistergos KE, Detrick B, Conte JV, McNiece I, Steenbergen C, Lardo AC, Hare JM Autologous mesenchymal stem cells produce reverse remodelling in chronic ischaemic cardiomyopathy. Eur. Heart J., 2009-07-08;30(22):2722-32. 2009-07-08 [PMID: 19586959] (Porcine)		Porcine
Wall R, Ross RP, Shanahan F, O'Mahony L, O'Mahony C, Coakley M, Hart O, Lawlor P, Quigley EM, Kiely B, Fitzgerald GF, Stanton C Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues. Am. J. Clin. Nutr., 2009-04-08;89(5):1393-401. 2009-04-08 [PMID: 19357220] (Porcine)		Porcine
Luna CM, Bruno DA, Garcia-Morato J, Mann KC, Risso Patron J, Sagardia J, Absi R, Garcia Bottino M, Marchetti D, Famiglietti A, Baleztena M, Biancolini C Effect of linezolid compared with glycopeptides in methicillin-resistant Staphylococcus aureus severe pneumonia in piglets. Chest, 2009-01-13;135(6):1564-71. 2009-01-13 [PMID: 19141525] (Porcine)		Porcine
Thorgersen EB, Pharo A, Haverson K, Axelsen AK, Gaustad P, Kotwal GJ, Sfyroera G, Mollnes TE Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood. Infect. Immun., 2008-12-01;77(2):725-32. 2008-12-01 [PMID: 19047409] (Porcine)		Porcine
Heinzel FR, Gres P, Boengler K, Duschin A, Konietzka I, Rassaf T, Snedovskaya J, Meyer S, Skyschally A, Kelm M, Heusch G, Schulz R Inducible nitric oxide synthase expression and cardiomyocyte dysfunction during sustained moderate ischemia in pigs. Circ. Res., 2008-09-25;103(10):1120-7. 2008-09-25 [PMID: 18818404] (Porcine)		Porcine
Kruse R, Essen-Gustavsson B, Fossum C, Jensen-Waern M Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery. Acta Vet. Scand., 2008-08-12;50(0):32. 2008-08-12 [PMID: 18700003] (Porcine)		Porcine
Sibila O, Luna CM, Agusti C, Baquero S, Gando S, Patron JR, Morato JG, Absi R, Bassi N, Torres A Effects of glucocorticoids in ventilated piglets with severe pneumonia. Eur. Respir. J., 2008-05-28;32(4):1037-46. 2008-05-28 [PMID: 18508831] (Porcine)		Porcine
Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model. J. Immunol., 2008-05-01;180(9):6346-53. 2008-05-01 [PMID: 18424758] (Porcine)		Porcine
Shimoda M, Iwasaki Y, Okada T, Sawada T, Kubota K Protective effect of Sivelestat in a porcine hepatectomy model prepared using an intermittent Pringle method. Eur. J. Pharmacol., 2008-02-29;587(1):248-52. 2008-02-29 [PMID: 18374331] (Porcine)		Porcine
Nold MF, Nold-Petry CA, Fischer D, Richter B, Blaheta R, Pfeilschifter J, Muhl H, Schranz D, Veldman A Activated protein C downregulates p38 mitogen-activated protein kinase and improves clinical parameters in an in-vivo model of septic shock. Thromb. Haemost., 2007-11-01;98(5):1118-26. 2007-11-01 [PMID: 18000619] (Porcine)		Porcine
Sibila O, Agusti C, Torres A, Baquero S, Gando S, Patron JR, Morato JG, Goffredo DH, Bassi N, Luna CM Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response. Eur. Respir. J., 2007-09-05;30(6):1167-72. 2007-09-05 [PMID: 17804447] (Porcine)		Porcine
Yano T, Itoh Y, Matsuo M, Kawashiri T, Egashira N, Oishi R Involvement of both tumor necrosis factor-alpha-induced necrosis and p53-mediated caspase-dependent apoptosis in nephrotoxicity of cisplatin. Apoptosis, 2007-10-01;12(10):1901-9. 2007-10-01 [PMID: 17701360] (Porcine)		Porcine
Luna CM, Baquero S, Gando S, Patron JR, Morato JG, Sibila O, Absi R, Famiglietti A, Vay CA, Von Stecher F, Agusti C, Torres A Experimental severe Pseudomonas aeruginosa pneumonia and antibiotic therapy in piglets receiving mechanical ventilation. Chest, 2007-08-01;132(2):523-31. 2007-08-01 [PMID: 17699131] (Porcine)		Porcine
Roesner JP, Petzelbauer P, Koch A, Mersmann J, Zacharowski PA, Boehm O, Reingruber S, Pasteiner W, Mascher D, Wolzt M, Barthuber C, Noldge-Schomburg GE, Scheeren TW, Zacharowski K The fibrin-derived peptide Bbeta15-42 is cardioprotective in a pig model of myocardial ischemia-reperfusion injury. Crit. Care Med., 2007-07-01;35(7):1730-5. 2007-07-01 [PMID: 17522584] (Porcine)		Porcine
Skyschally A, Gres P, Hoffmann S, Haude M, Erbel R, Schulz R, Heusch G Bidirectional role of tumor necrosis factor-alpha in coronary microembolization: progressive contractile dysfunction versus delayed protection against infarction. Circ. Res., 2006-12-14;100(1):140-6. 2006-12-14 [PMID: 17170366] (Porcine)		Porcine
Moller S, Lauridsen C Dietary fatty acid composition rather than vitamin E supplementation influence ex vivo cytokine and eicosanoid response of porcine alveolar macrophages. Cytokine, 2006-08-17;35(1):6-12. 2006-08-17 [PMID: 16914322] (Porcine)		Porcine
Zhu XY, Daghini E, Chade AR, Rodriguez-Porcel M, Napoli C, Lerman A, Lerman LO Role of oxidative stress in remodeling of the myocardial microcirculation in hypertension. Arterioscler. Thromb. Vasc. Biol., 2006-05-18;26(8):1746-52. 2006-05-18 [PMID: 16709946] (Porcine)		Porcine
Zhu T, Sennlaub F, Beauchamp MH, Fan L, Joyal JS, Checchin D, Nim S, Lachapelle P, Sirinyan M, Hou X, Bossolasco M, Rivard GE, Heveker N, Chemtob S Proangiogenic effects of protease-activated receptor 2 are tumor necrosis factor-alpha and consecutively Tie2 dependent. Arterioscler. Thromb. Vasc. Biol., 2006-01-26;26(4):744-50. 2006-01-26 [PMID: 16439712] (Porcine)		Porcine
Changhua L, Jindong Y, Defa L, Lidan Z, Shiyan Q, Jianjun X Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide. J. Nutr., 2005-02-01;135(2):239-44. 2005-02-01 [PMID: 15671220] (Porcine)		Porcine
Royaee AR, Husmann RJ, Dawson HD, Calzada-Nova G, Schnitzlein WM, Zuckermann FA, Lunney JK Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccination. Vet. Immunol. Immunopathol., 2004-12-08;102(3):199-216. 2004-12-08 [PMID: 15507306] (Porcine)		Porcine
Glew PA, Campher MJ, Pearson K, Schofield JC, Davey AK The effect of warm humidified CO2 on the dissipation of residual gas following laparoscopy in piglets. J Am Assoc Gynecol Laparosc, 2004-05-01;11(2):204-10. 2004-05-01 [PMID: 15200776] (Porcine)		Porcine
Majetschak M, Krehmeier U, Bardenheuer M, Denz C, Quintel M, Voggenreiter G, Obertacke U Extracellular ubiquitin inhibits the TNF-alpha response to endotoxin in peripheral blood mononuclear cells and regulates endotoxin hyporesponsiveness in critical illness. Blood, 2002-10-24;101(5):1882-90. 2002-10-24 [PMID: 12406900] (Porcine)		Porcine
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FAQs for TNF-alpha (PTA00). (Showing 1 - 4 of 4 FAQs).

I need to know about TNF-alpha antibody kits for mice.
- We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
- I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
- The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
- I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ... Read full blog post.

Immune Cell Metabolic Flux Influences Type I Diabetes
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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit... Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven of the Stanford University, CA, explained the relationship between four diseases:... Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN... Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t... Read full blog post.

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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

TNF-alpha [Unconjugated]
210-TA-005

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Porcine TNF-alpha Quantikine ELISA Kit

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Publications for TNF-alpha (PTA00)(85)

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Product General Protocols

FAQs for TNF-alpha (PTA00). (Showing 1 - 4 of 4 FAQs).

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